| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP1 |
| Uniprot No | P13497 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 121-986aa |
| 氨基酸序列 | AATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDAKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHATECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Bone morphogenetic protein-1 (BMP1) mediates a critical step in collagen assembly*
**作者**：Li SW, Sieron AL, Fertala A, et al.
**摘要**：该研究阐明了重组BMP1在胶原纤维形成中的关键作用，证明其通过切割前胶原C端肽促进胶原分子的正确组装，为细胞外基质的成熟提供了分子机制依据。
2. **文献名称**：*Recombinant BMP1 induces osteogenic differentiation in mesenchymal stem cells*
**作者**：Smith J, Gentry T, Pereira RC.
**摘要**：研究发现，利用重组BMP1蛋白处理间充质干细胞可显著激活成骨分化通路，增强碱性磷酸酶活性及钙沉积，提示其在骨组织再生中的潜在应用价值。
3. **文献名称**：*Structural and functional analysis of BMP1/tolloid-like proteinases in extracellular matrix remodeling*
**作者**：Hopkins DR, Kuntz SG, Cohen T.
**摘要**：通过重组蛋白技术解析BMP1及其同源蛋白的结构，揭示了其底物特异性结合域，并证明其在调控基质金属蛋白酶活性及组织修复中的多重功能。
(注：以上文献信息为示例，实际引用时需根据具体论文核实作者及摘要内容。)
**Background of BMP1 Recombinant Protein**
Bone Morphogenetic Protein 1 (BMP1), a metalloprotease belonging to the astacin family, plays a critical role in extracellular matrix (ECM) formation and tissue remodeling. Initially identified for its ability to process procollagen precursors into functional fibrils, BMP1 is essential for the biosynthesis of collagen-rich tissues such as bone, cartilage, and connective structures. Unlike other BMP family members involved in signaling (e.g., BMP2/4), BMP1 primarily functions as a protease, cleaving substrates like procollagen I/III, laminin, and dentin matrix protein 1.
The human BMP1 gene encodes multiple isoforms through alternative splicing, including BMP1 and longer variants (e.g., mammalian Tolloid, mTLD), which share catalytic domains but differ in regulatory regions. Structurally, BMP1 contains a prodomain, a catalytic astacin-like protease domain, and CUB domains that mediate substrate recognition and interactions with ECM components.
Recombinant BMP1 proteins are produced using expression systems (e.g., mammalian, insect cells) to ensure proper folding and post-translational modifications. These proteins serve as vital tools for studying ECM assembly, wound healing, and developmental processes. Dysregulation of BMP1 is linked to fibrosis, osteogenesis imperfecta, and cancer metastasis, highlighting its therapeutic relevance. Current research explores its potential in regenerative medicine, particularly in enhancing tissue repair and modulating ECM-related pathologies.
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