| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNNT3 |
| Uniprot No | P45378 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-269aa |
| 氨基酸序列 | SDEEVEQVE EQYEEEEEAQ EEAAEVHEEV HEPEEVQEDT AEEDAEEEKP RPKLTAPKIP EGEKVDFDDI QKKRQNKDLM ELQALIDSHF EARKKEEEEL VALKERIEKR RAERAEQQRI RAEKERERQN RLAEEKARRE EEDAKRRAED DLKKKKALSS MGANYSSYLA KADQKRGKKQ TAREMKKKIL AERRKPLNID HLGEDKLRDK AKELWETLHQ LEIDKFEFGE KLKRQKYDIT TLRSRIDQAQ KHSKKAGTPA KGKVGGRWK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNNT3重组蛋白的3篇代表性文献摘要(注：文献为示例性概括，具体内容需根据实际研究调整)：
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1. **文献名称**: *"Expression and Purification of Recombinant Human Fast Skeletal Troponin T (TNNT3) in Escherichia coli"*
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了利用大肠杆菌表达系统高效表达人源TNNT3重组蛋白的优化方法，通过His标签亲和层析纯化获得高纯度蛋白。实验验证了重组TNNT3与肌钙蛋白复合体其他亚基(TNNC和TNNI)的体外结合能力，为后续研究其结构与功能奠定了基础。
2. **文献名称**: *"Functional Analysis of TNNT3 Mutations Linked to Distal Arthrogryposis"*
**作者**: Sung SS, et al.
**摘要**: 文章通过重组TNNT3蛋白结合体外肌动球蛋白实验，揭示了与远端关节挛缩症相关的基因突变(如R63H和R133W)如何破坏肌钙蛋白复合体的钙离子敏感性，导致骨骼肌收缩功能障碍，为疾病机制提供了分子水平解释。
3. **文献名称**: *"Structural Insights into TNNT3 Dynamics During Muscle Contraction"*
**作者**: Li X, et al.
**摘要**: 利用重组TNNT3蛋白的X射线晶体学分析，阐明了其在肌肉收缩过程中构象变化的分子机制，发现其N端结构域在调控肌钙蛋白-原肌球蛋白相互作用中起关键作用，为靶向药物设计提供了结构基础。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“TNNT3 recombinant protein”或结合疾病名称(如“distal arthrogryposis”)筛选近期研究。
TNNT3. or troponin T3. is a protein encoded by the TNNT3 gene and serves as the fast skeletal muscle isoform of troponin T, a critical component of the troponin complex. This complex regulates calcium-dependent skeletal muscle contraction by modulating interactions between actin and myosin filaments. TNNT3 specifically binds to tropomyosin, anchoring the troponin complex to thin filaments and facilitating conformational changes during muscle activation. Its expression is predominantly observed in fast-twitch muscle fibers, which are responsible for rapid, forceful movements.
Recombinant TNNT3 protein is produced using biotechnological platforms, such as bacterial (E. coli) or mammalian expression systems, enabling large-scale production with high purity. The recombinant form retains the functional properties of native TNNT3. including calcium-sensitive binding to other troponin subunits (TnI and TnC) and tropomyosin. This makes it valuable for structural studies, drug screening, and understanding molecular mechanisms of muscle contraction.
Research on TNNT3 has clinical relevance, as mutations in TNNT3 are linked to distal arthrogryposis syndromes, congenital disorders characterized by joint contractures and muscle weakness. Recombinant TNNT3 aids in studying these pathogenic variants, elucidating their impact on protein stability and calcium signaling. Additionally, it serves as an antigen in autoimmune myopathy diagnostics and a tool for developing therapeutic strategies targeting muscle dysfunction. Recent advances in cryo-EM and X-ray crystallography have further leveraged recombinant TNNT3 to resolve structural details of the troponin-tropomyosin regulatory system, enhancing our understanding of striated muscle physiology and pathology.
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