| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COQ10B |
| Uniprot No | Q9H8M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-238aa |
| 氨基酸序列 | ASC GILMSRTLPL HTSILPKEIC ARTFFKITAP LINKRKEYSE RRILGYSMQE MYDVVSGVED YKHFVPWCKK SDVISKRSGY CKTRLEIGFP PVLERYTSVV TLVKPHLVKA SCTDGRLFNH LETIWRFSPG LPGYPRTCTL DFSISFEFRS LLHSQLATLF FDEVVKQMVA AFERRACKLY GPETNIPREL MLHEVHHT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COQ10B重组蛋白研究的虚构示例参考文献(仅供示例参考，实际文献请查询权威数据库)：
1. **《Functional Characterization of Recombinant COQ10B Protein in Mitochondrial Electron Transport》**
- 作者：Smith, J., et al.
- 摘要：研究通过大肠杆菌表达系统成功纯化重组COQ10B蛋白，证实其在体外可恢复辅酶Q缺陷型细胞模型的电子传递链功能，提示COQ10B在辅酶Q合成中的关键作用。
2. **《Structural Analysis of Human COQ10B Using Recombinant Expression and X-ray Crystallography》**
- 作者：Zhang, L., et al.
- 摘要：首次解析了重组人源COQ10B蛋白的晶体结构，揭示其与辅酶Q结合域的关键氨基酸残基，为靶向药物设计提供结构基础。
3. **《Recombinant COQ10B Ameliorates Oxidative Stress in a Mouse Model of Cardiomyopathy》**
- 作者：Tanaka, K., et al.
- 摘要：通过腺病毒载体递送重组COQ10B蛋白至心肌病模型小鼠，显著减少线粒体氧化损伤并改善心脏功能，表明其潜在治疗价值。
4. **《Optimization of COQ10B Recombinant Production in Yeast for Industrial Applications》**
- 作者：Müller, R., et al.
- 摘要：开发了一种酿酒酵母高效表达重组COQ10B的工艺，通过发酵条件优化使蛋白产量提升5倍，为规模化生产奠定基础。
**注意**：以上文献为示例，实际研究需通过PubMed、Google Scholar或Web of Science等平台检索真实文献(关键词：COQ10B, recombinant protein, Coenzyme Q10 biosynthesis)。
**Background of COQ10B Recombinant Protein**
COQ10B (Coenzyme Q-binding protein COQ10 homolog B) is a mitochondrial protein implicated in the biosynthesis and regulation of coenzyme Q10 (ubiquinone), a vital lipid molecule essential for electron transport in the mitochondrial respiratory chain and cellular antioxidant defense. COQ10B, a homolog of yeast COQ10. is part of a multi-protein complex involved in the final steps of coenzyme Q10 synthesis, particularly its stabilization and functional maturation.
Structurally, COQ10B contains a conserved steroidogenic acute regulatory protein (StAR)-related lipid-transfer (START) domain, suggesting a role in lipid binding or transport. It localizes to the mitochondrial inner membrane, where it interacts with other COQ proteins to maintain coenzyme Q10 homeostasis. Dysregulation of COQ10B is linked to mitochondrial disorders, neurodegenerative diseases, and aging-related pathologies due to impaired energy production and oxidative stress.
Recombinant COQ10B protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form retains biochemical activity, allowing researchers to explore its molecular interactions, enzymatic roles, and therapeutic potential. Purified COQ10B is utilized in *in vitro* assays, structural studies, and drug screening aimed at addressing coenzyme Q10 deficiencies or mitochondrial dysfunction.
Research on COQ10B also extends to its diagnostic relevance, as mutations in the COQ10B gene are associated with rare coenzyme Q10 deficiency syndromes. Recombinant protein tools are critical for elucidating disease mechanisms and developing targeted therapies to restore mitochondrial function.
In summary, COQ10B recombinant protein serves as a key reagent for dissecting coenzyme Q10 biology, advancing translational studies in metabolic and degenerative diseases, and fostering innovations in mitochondrial medicine.
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