| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN1 |
| Uniprot No | P07384 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-714aa |
| 氨基酸序列 | MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA |
| 预测分子量 | 88.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于CAPN1重组蛋白的参考文献示例(请注意这些文献为虚构，仅供格式参考)：
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1. **文献名称**：*Heterologous Expression and Functional Characterization of Recombinant CAPN1 in Insect Cells*
**作者**：Chen, L., et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统成功表达了重组人源CAPN1蛋白，并通过钙离子依赖性活性实验验证其酶学功能，为后续结构解析提供了高纯度蛋白样本。
2. **文献名称**：*Optimization of CAPN1 Recombinant Protein Production in E. coli for Drug Screening*
**作者**：Garcia, R., et al.
**摘要**：通过优化大肠杆菌表达条件(如诱导温度、IPTG浓度)，显著提高了CAPN1重组蛋白的溶解度与产量，并应用于高通量抑制剂筛选平台开发。
3. **文献名称**：*Cryo-EM Structure of CAPN1 Reveals Calcium-Induced Conformational Changes*
**作者**：Wang, Y., et al.
**摘要**：利用重组CAPN1蛋白进行冷冻电镜分析，揭示了钙离子结合后引起的构象变化，阐明了其自抑制状态解除的分子机制。
4. **文献名称**：*Role of Recombinant CAPN1 in Tau Protein Cleavage: Implications for Alzheimer’s Disease*
**作者**：Kumar, S., et al.
**摘要**：体外实验表明，重组CAPN1可切割tau蛋白生成神经毒性片段，提示其在阿尔茨海默病病理中的作用，为靶向治疗提供依据。
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**注意**：以上文献为模拟生成，实际研究中请通过PubMed、Web of Science等数据库检索真实文献(关键词：CAPN1 recombinant protein, μ-calpain expression)。
**Background of CAPN1 Recombinant Protein**
Calpain-1 (CAPN1), a member of the calpain family of calcium-dependent cysteine proteases, plays a critical role in modulating cellular processes such as signal transduction, apoptosis, cytoskeletal remodeling, and cell migration. It exists as a heterodimer composed of a large catalytic subunit (encoded by the *CAPN1* gene) and a small regulatory subunit (CAPNS1). CAPN1 requires calcium for activation, which induces conformational changes to expose its proteolytic site. Dysregulation of CAPN1 has been linked to pathologies including neurodegenerative disorders, muscular dystrophy, and cancer, underscoring its importance in both physiological and disease contexts.
Recombinant CAPN1 protein is produced using biotechnological methods, typically through expression in bacterial (e.g., *E. coli*) or mammalian systems. This engineered protein retains enzymatic activity and structural integrity, enabling researchers to study its mechanisms, interactions, and regulatory pathways *in vitro*. Purification techniques like affinity chromatography ensure high purity and functionality.
The availability of recombinant CAPN1 has advanced drug discovery, particularly in screening inhibitors for conditions like Alzheimer’s and ischemia-reperfusion injury. It also aids in deciphering calpain’s role in cellular stress responses and post-translational modifications. However, challenges remain in mimicking its native activation environment due to calcium sensitivity and autodegradation. Ongoing research focuses on optimizing recombinant variants for stability and therapeutic targeting, positioning CAPN1 as a promising biomarker and intervention point in multiple diseases.
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