| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMKK2 |
| Uniprot No | Q96RR4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-588aa |
| 氨基酸序列 | SSCVSSQPS SNRAAPQDEL GGRGSSSSES QKPCEALRGL SSLSIHLGME SFIVVTECEP GCAVDLGLAR DRPLEADGQE VPLDTSGSQA RPHLSGRKLS LQERSQGGLA AGGSLDMNGR CICPSLPYSP VSSPQSSPRL PRRPTVESHH VSITGMQDCV QLNQYTLKDE IGKGSYGVVK LAYNENDNTY YAMKVLSKKK LIRQAGFPRR PPPRGTRPAP GGCIQPRGPI EQVYQEIAIL KKLDHPNVVK LVEVLDDPNE DHLYMVFELV NQGPVMEVPT LKPLSEDQAR FYFQDLIKGI EYLHYQKIIH RDIKPSNLLV GEDGHIKIAD FGVSNEFKGS DALLSNTVGT PAFMAPESLS ETRKIFSGKA LDVWAMGVTL YCFVFGQCPF MDERIMCLHS KIKSQALEFP DQPDIAEDLK DLITRMLDKN PESRIVVPEI KLHPWVTRHG AEPLPSEDEN CTLVEVTEEE VENSVKHIPS LATVILVKTM IRKRSFGNPF EGSRREERSL SAPGNLLTKK PTRECESLSE LKEARQRRQP PGHRPAPRGG GGSALVRGSP CVESCWAPAP GSPARMHPLR PEEAMEPE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Characterization of recombinant CaMKK2 and its regulation by phosphorylation"**
- Author: Racioppi L, et al.
- 摘要：研究报道了重组人源CAMKK2在大肠杆菌中的表达与纯化，分析了其激酶活性及通过磷酸化调控的机制，揭示了其与AMPK信号通路的相互作用。
2. **"Structural and functional analysis of CAMKK2 identifies a regulatory kinase domain"**
- Author: Tokumitsu H, et al.
- 摘要：通过X射线晶体学解析了重组CAMKK2的激酶结构域三维结构，揭示了其自抑制构象及钙/钙调素依赖的激活机制，为靶向药物设计提供依据。
3. **"CAMKK2 regulates mitochondrial function in cancer cells via AMPK-independent pathways"**
- Author: Anderson KA, et al.
- 摘要：利用重组CAMKK2蛋白进行体外功能实验，发现其通过非AMPK依赖途径调控肿瘤细胞线粒体代谢，提示其在癌症治疗中的潜在作用。
4. **"Development of a high-throughput assay for CAMKK2 inhibitors using recombinant protein"**
- Author: Scott JW, et al.
- 摘要：研究建立了基于重组CAMKK2蛋白的高通量筛选平台，用于鉴定新型小分子抑制剂，并验证其对肥胖相关代谢疾病的治疗效果。
**Background of CAMKK2 Recombinant Protein**
Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) is a serine/threonine kinase that acts as a key upstream regulator of AMP-activated protein kinase (AMPK) and calcium/calmodulin-dependent protein kinase IV (CAMKIV). It plays a central role in intracellular calcium signaling pathways, linking cellular energy status and calcium fluxes to downstream metabolic and transcriptional responses. CAMKK2 is highly expressed in the brain, liver, and testes, and its activity is implicated in processes such as glucose homeostasis, neuronal survival, and autophagy.
Recombinant CAMKK2 protein is produced via genetic engineering, typically using expression systems like *E. coli*, insect cells, or mammalian cells. This engineered protein retains the enzymatic activity of native CAMKK2. enabling researchers to study its structure, function, and interactions in controlled settings. Its recombinant form often includes tags (e.g., His-tag, GST) for simplified purification and detection.
Research involving CAMKK2 recombinant protein has shed light on its role in diseases. Overactivation of CAMKK2 is associated with prostate cancer progression, obesity, and metabolic syndrome, while its inhibition shows therapeutic potential in preclinical models. Additionally, CAMKK2-AMPK signaling is explored for neuroprotection in Alzheimer’s and Parkinson’s diseases.
The protein is widely used in *in vitro* kinase assays to identify inhibitors or activators, aiding drug discovery. Its recombinant form also serves as a tool to dissect calcium-mediated signaling cascades and validate biomarkers. Despite its promise, challenges remain in achieving tissue-specific targeting and minimizing off-effects in therapeutic applications.
Overall, CAMKK2 recombinant protein is a vital resource for unraveling the molecular mechanisms of calcium-dependent signaling and advancing translational research in metabolism, oncology, and neurology.
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