| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF558 |
| Uniprot No | Q96NG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402 aa |
| 活性数据 | MAAVILPSTAAPSSLFPASQQKGHTQGGELVNELLTSWLRGLVTFEDVAVEFTQEEWALLDPAQRTLYRDVMLENCRNLASLGCRVNKPSLISQLEQDKKVVTEERGILPSTCPDLETLLKAKWLTPKKNVFRKEQSKGVKTERSHRGVKLNECNQCFKVFSTKSNLTQHKRIHTGEKPYDCSQCGKSFSSRSYLTIHKRIHNGEKPYECNHCGKAFSDPSSLRLHLRIHTGEKPYECNQCFHVFRTSCNLKSHKRIHTGENHHECNQCGKAFSTRSSLTGHNSIHTGEKPYECHDCGKTFRKSSYLTQHVRTHTGEKPYECNECGKSFSSSFSLTVHKRIHTGEKPYECSDCGKAFNNLSAVKKHLRTHTGEKPYECNHCGKSFTSNSYLSVHKRIHNRWI |
| 分子量 | 72.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF558蛋白的参考文献摘要(均为示例性文献，实际引用需以真实论文为准)：
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1. **文献名称**：*ZNF558 regulates transcriptional activation in primate-specific innate immune responses*
**作者**：Johansson, P.A., et al.
**摘要**：研究发现ZNF558作为灵长类特异性锌指蛋白，通过重组蛋白实验证实其与特定DNA启动子区域结合，调控干扰素通路基因表达，可能参与物种特异性免疫应答。
2. **文献名称**：*Structural analysis of recombinant ZNF558 reveals its role in chromatin remodeling*
**作者**：Chen, L., & Zhang, Y.
**摘要**：通过体外表达并纯化重组ZNF558蛋白，解析其三维结构，揭示其锌指结构域通过招募组蛋白修饰酶调控染色质凝缩，影响细胞分化。
3. **文献名称**：*Functional characterization of ZNF558 in neural progenitor cells using recombinant protein assays*
**作者**：Müller, T., et al.
**摘要**：利用重组ZNF558蛋白进行体外DNA亲和实验，证明其在神经干细胞中结合SOX基因启动子，抑制神经元过早分化，维持干细胞多能性。
4. **文献名称**：*ZNF558 interacts with KRAB-associated protein 1 (KAP1) via recombinant co-immunoprecipitation*
**作者**：Kim, S., & Lee, J.
**摘要**：通过重组蛋白共沉淀实验发现ZNF558与KAP1形成复合物，协同抑制逆转录转座子活性，可能在基因组稳定性中起关键作用。
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**注**：上述文献为示例性质，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。研究ZNF558的重组蛋白多聚焦于其DNA结合特性、结构解析及调控机制。
ZNF558 (Zinc Finger Protein 558) is a transcription factor belonging to the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which is characterized by tandem arrays of C2H2-type zinc finger motifs. These proteins typically function in gene regulation by binding to specific DNA sequences. ZNF558 is evolutionarily conserved in primates and plays a role in transcriptional repression, often interacting with chromatin-modifying complexes to influence epigenetic states.
Notably, ZNF558 gained attention for its role in mitochondrial regulation. Studies revealed its involvement in controlling the expression of *SPATA18* (also called *PSPH*), a gene linked to mitochondrial quality control and mitophagy. Interestingly, ZNF558 itself is regulated by VNTR (variable number tandem repeat) sequences, highlighting its connection to genetic variability in humans.
In neurodevelopment, ZNF558 has been implicated in cortical expansion and neuron differentiation, with reduced expression observed in certain neurodevelopmental disorders. Its regulatory impact on *LIN28A*, a gene critical for brain size evolution, suggests a role in primate-specific traits. Additionally, ZNF558 has been associated with pathologies such as Huntington’s disease and cancer, though its precise mechanisms remain under investigation.
Recombinant human ZNF558 protein is commonly produced in vitro for functional studies, enabling research into its DNA-binding specificity, protein interactions, and therapeutic potential. Its dual roles in mitochondrial function and transcriptional regulation make it a compelling target for understanding cellular homeostasis and disease pathways.
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