| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF524 |
| Uniprot No | Q96C55 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264 aa |
| 活性数据 | MDTPSPDPLPSPLPGEEEKPLALSPPVPRGRRGRRPGGATSSNRTLKASLHRKRGRPPKSGQEPPLVQVQGVTAPVGSSGGSDLLLIDDQGVPYTVSEGSAAGPEGSGPRKAPHFCPVCLRAFPYLSDLERHSISHSELKPHQCKVCGKTFKRSSHLRRHCNIHAGLRPFRCPLCTRRFREAGELAHHHRVHSGERPYQCPICRLRFTEANTLRRHAKRKHPEAMGVPLCAPDPGSEPPWDEEGIPATAGAEEEEETEGKGEPA |
| 分子量 | 55.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF524蛋白的3-4条参考文献示例，格式为文献名称、作者及摘要概括：
1. **Structural Insights into the DNA-Binding Mechanism of Human ZNF524 Protein**
*Li, X. et al. (2020)*
摘要：通过X射线晶体学解析了ZNF524蛋白的锌指结构域与特定DNA序列结合的分子机制，揭示其通过保守残基识别DNA双螺旋结构，可能参与调控基因转录。
2. **ZNF524 Regulates Pluripotency in Embryonic Stem Cells via Epigenetic Modulation**
*Wang, Y. et al. (2018)*
摘要：研究发现ZNF524在维持胚胎干细胞多能性中起关键作用，通过染色质免疫沉淀测序(ChIP-seq)鉴定其结合靶基因启动子，调控组蛋白修饰和干性相关基因表达。
3. **Comprehensive Analysis of Zinc Finger Proteins in Human Genomes: Functional Clues on ZNF524**
*Smith, J. & Johnson, R. (2015)*
摘要：综述性研究总结了锌指蛋白家族(包括ZNF524)的结构分类及潜在功能，提出ZNF524可能参与发育调控和疾病相关信号通路。
4. **Aberrant Expression of ZNF524 in Colorectal Cancer and Its Prognostic Implications**
*Chen, H. et al. (2022)*
摘要：基于临床样本分析，发现ZNF524在结直肠癌组织中表达显著下调，其低表达与患者生存率降低相关，体外实验证实其过表达抑制癌细胞增殖和迁移。
(注：以上文献为示例，实际研究请通过学术数据库核实。)
Zinc finger protein 524 (ZNF524) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C₂H₂-type zinc finger motifs that enable sequence-specific DNA binding. These proteins are primarily involved in transcriptional regulation, often acting as repressors by recruiting chromatin-modifying complexes via their KRAB domains. ZNF524. though less studied compared to other ZNFs, is hypothesized to play roles in gene silencing, embryonic development, and cellular differentiation. Its expression appears tissue-specific, with notable presence in reproductive and neural tissues. Recombinant ZNF524 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Current research focuses on elucidating its target genes, mechanistic interactions with DNA or protein partners, and potential links to diseases such as cancers or developmental disorders. However, its precise biological functions and regulatory networks remain poorly characterized, underscoring the need for further biochemical and genomic investigations. Recombinant ZNF524 serves as a critical tool for in vitro assays, structural studies, and deciphering its role in epigenetic regulation or transcriptional landscapes.
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