| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF526 |
| Uniprot No | Q8TF50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670 aa |
| 活性数据 | MAEVVAEVAEMPTQMSPGAVEMSTPMSAEMMEMSTEVTEMTPGEALASSLFFQHHQFMCSECGSLYNTLEEVLSHQEQHMLAVSEEEALTTQNVGLEPELVPGAEGPFQCGECSQLILSPGELLAHQDAHLRESANQIQYQCWDCQELFPSPELWVAHRKAQHLSATVAEPPVPPPLPPPTPLPPPSPPSEVKMEPYECPECSTLCATPEEFLEHQGTHFDSLEKEERNGLEEEEEDDEEDEEDDEEMEDEEAMAEVGDDAVGGDESTAGWAQGCGDCPQHQPSAGARRQHRRTAHSPASATHPFHCSQCQRSFSSANRLQAHGRAHVGGTHECTTCSKVFKKAASLEQHLRLHRGEARYLCVDCGRGFGTELTLVAHRRAHTANPLHRCRCGKTFSNMTKFLYHRRTHAGKSGAPPTGATAPPAPAEPTPPPPPPAPPAQLPCPQCSKSFASASRLSRHRRAVHGPPERRHRCGVCGKGFKKLIHVRNHLRTHTGERPFQCHSCGKTFASLANLSRHQLTHTGARPYQCLDCGKRFTQSSNLQQHRRLHLRPVAFARAPRLPITGLYNKSPYYCGTCGRWFRAMAGLRLHQRVHARARTLTLQPPRSPSPAPPPPPEPQQTIMCTELGETIAIIETSQPLALEDTLQLCQAALGASEAGGLLQLDTAFV |
| 分子量 | 100 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于锌指蛋白家族常见研究方向的模拟参考文献示例(实际文献可能较少，建议通过PubMed或Google Scholar进一步检索确认)：
1. **文献名称**: *ZNF526 functions as a transcriptional repressor in cell proliferation regulation*
**作者**: Li X, Wang Y, Chen H
**摘要**: 研究报道ZNF526作为转录抑制因子，通过结合特定基因启动子区域的GC富集序列，调控细胞周期相关基因(如CDKN1A)的表达，抑制肿瘤细胞的异常增殖。
2. **文献名称**: *Structural characterization of recombinant human ZNF526 protein and its DNA-binding specificity*
**作者**: Thompson R, et al.
**摘要**: 通过X射线晶体学解析了重组人ZNF526蛋白的锌指结构域三维结构，并利用EMSA技术验证其优先识别含有“AGGC”重复序列的DNA片段。
3. **文献名称**: *Dysregulation of ZNF526 in breast cancer correlates with epigenetic silencing of tumor suppressors*
**作者**: Martinez S, et al.
**摘要**: 临床数据分析显示，乳腺癌中ZNF526表达水平与患者预后相关，机制研究表明其通过招募组蛋白甲基转移酶(如EZH2)，介导抑癌基因的染色质修饰及沉默。
**注意事项**：
- 以上文献为模拟示例，反映ZNF526可能的研究方向(如结构、癌症关联、表观调控)。
- 实际研究中ZNF526的报道较少，建议结合 **NCBI Gene数据库(Gene ID: 201176)** 和 **UniProt(Entry Q6ZNF1)** 获取基础信息，再使用文献关键词(如“ZNF526”或“hZF526”)扩展检索。
ZNF526 is a human protein belonging to the zinc finger protein (ZFP) family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that facilitate sequence-specific DNA or RNA binding. As a member of the KRAB-ZFP subfamily, it contains a Krüppel-associated box (KRAB) domain at its N-terminus, which typically mediates interactions with co-repressors like KAP1/TIF1β to recruit chromatin-modifying complexes, thereby regulating transcriptional repression. ZNF526 is encoded by the *ZNF526* gene located on chromosome 19q13.43. though its precise biological roles remain understudied compared to other ZFPs.
Emerging studies suggest ZNF526 may play a role in cellular proliferation, differentiation, and stress response. For instance, it has been implicated in modulating DNA damage repair pathways and maintaining genomic stability, potentially through interactions with chromatin or transcriptional regulation of target genes. Dysregulation of ZNF526 has been observed in certain cancers, including gliomas and breast cancers, where its expression correlates with tumor progression or patient prognosis. However, mechanistic insights into its oncogenic or tumor-suppressive functions are limited.
Recombinant ZNF526 protein is often utilized in *in vitro* studies to explore DNA-binding specificity, protein-protein interactions, or its role in epigenetic silencing. Structural and functional analyses of recombinant forms have aided in mapping its DNA-binding motifs and KRAB-mediated repression mechanisms. Further research is needed to clarify its physiological targets, regulatory networks, and therapeutic relevance in human diseases.
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