| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF529 |
| Uniprot No | Q6P280 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458 aa |
| 活性数据 | MESSKLCGLEGSIFRNDWQSKSKIDLQGPEVGYFSQMKIISENVPSYKTHESLTLPRRTHDSEKPYEYKEYEKVFSCDLEFDEYQKIHTGGKNYECNQCWKTFGIDNSSMLQLNIHTGVKPCKYMEYGNTCSFYKDFNVYQKIHNEKFYKCKEYRRTFERVGKVTPLQRVHDGEKHFECSFCGKSFRVHAQLTRHQKIHTDEKTYKCMECGKDFRFHSQLTEHQRIHTGEKPYKCMHCEKVFRISSQLIEHQRIHTGEKPYACKECGKAFGVCRELARHQRIHTGKKPYECKACGKVFRNSSSLTRHQRIHTGEKPYKCKECEKAFGVGSELTRHERIHSGQKPYECKECGKFFRLTSALIQHQRIHSGEKPYECKVCGKAFRHSSALTEHQRIHTGEKPYECKACGKAFRHSSSFTKHQRIHTDDKPYECKECGNSFSVVGHLTCQPKIYTGEKSFD |
| 分子量 | 80.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF529蛋白的模拟参考文献示例(注：ZNF529研究较少，以下内容基于学术假设，实际文献需通过数据库验证)：
1. **文献名称**：*Functional Characterization of Recombinant ZNF529 in Cancer Cell Proliferation*
**作者**：Zhang, Y., et al. (2020)
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人ZNF529蛋白，发现其通过调控细胞周期相关基因(如CDKN1A)抑制肝癌细胞增殖，提示ZNF529可能具有肿瘤抑制功能。
2. **文献名称**：*Structural Insights into the DNA-Binding Motifs of ZNF529*
**作者**：Smith, J., et al. (2021)
**摘要**：通过X射线晶体学解析了ZNF529的C末端锌指结构域三维结构，揭示其保守的DNA结合位点，并验证了其在体外与特定启动子序列(如GC-rich区域)的结合能力。
3. **文献名称**：*CRISPR-Cas9 Knockout of ZNF529 Impairs Embryonic Stem Cell Differentiation*
**作者**：Lee, H., et al. (2019)
**摘要**：利用重组ZNF529蛋白恢复基因敲除的胚胎干细胞功能，证明其在胚胎发育中通过调控多能性基因(如OCT4)维持细胞分化平衡。
4. **文献名称**：*ZNF529 Interaction with NF-κB Signaling Pathway in Inflammatory Responses*
**作者**：Chen, L., et al. (2022)
**摘要**：通过重组ZNF529蛋白的免疫共沉淀实验，发现其与NF-κB亚基p65相互作用，负调控炎症因子(如TNF-α、IL-6)的表达，可能成为炎症疾病的治疗靶点。
**提示**：实际研究中ZNF529的相关文献有限，建议结合锌指蛋白家族(如ZNF384、ZNF263等)或利用生物信息学工具(如UniProt、STRING)挖掘其潜在功能。
ZNF529 is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, which represents one of the largest groups of transcriptional regulators in humans. Characterized by tandem C₂H₂-type zinc finger motifs, these proteins typically bind DNA sequences to modulate gene expression. The KRAB domain, located at the N-terminus of ZNF529. facilitates interactions with co-repressors like TRIM28/KAP1. recruiting chromatin-modifying complexes to induce transcriptional repression. While the exact biological role of ZNF529 remains under investigation, it is hypothesized to participate in developmental processes, cellular differentiation, or genome regulation, similar to related KRAB-ZFPs. Genomically, ZNF529 is located on chromosome 19. and its expression has been detected in various tissues, suggesting broad functional relevance. Interest in recombinant ZNF529 stems from its potential involvement in disease pathways, including cancer or neurodevelopmental disorders. Recombinant forms of ZNF529. produced in systems like HEK293 or Escherichia coli, enable in vitro studies to map DNA-binding specificity, protein-protein interactions, and regulatory mechanisms. Current research aims to identify its target genes, elucidate its role in epigenetic silencing, and explore therapeutic applications. Despite limited functional data, ZNF529 exemplifies the complexity of KRAB-ZFPs in balancing adaptive gene regulation and disease susceptibility.
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