| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF517 |
| Uniprot No | Q6ZMY9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492 aa |
| 活性数据 | MAMALPMPGPQEAVVFEDVAVYFTRIEWSCLAPDQQALYRDVMLENYGNLASLGFLVAKPALISLLEQGEEPGALILQVAEQSVAKASLCTDSRMEAGIMESPLQRKLSRQAGLPGTVWGCLPWGHPVGGHPAPPHPHGGPEDGSDKPTHPRAREHSASPRVLQEDLGRPVGSSAPRYRCMCGKAFRYNSLLLRHQIIHTGAKPFQCTECGKAFKQSSILLRHQLIHTEEKPFQCGECGKAFRQSTQLAAHHRVHTRERPYACGECGKAFSRSSRLLQHQKFHTGEKPFACTECGKAFCRRFTLNEHGRIHSGERPYRCLRCGQRFIRGSSLLKHHRLHAQEGAQDGGAGQGALLGAAQRPQAGDPPHECPVCGRPFRHNSLLLLHLRLHTGEKPFECAECGKAFGRKSNLTLHQKIHTKEKPFACTECGKAFRRSYTLNEHYRLHSGERPYRCRACGRACSRLSTLIQHQKVHGREPGEDTEGRRAPCWAS |
| 分子量 | 54.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF517蛋白的3篇代表性文献的简要信息，供参考：
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1. **文献名称**：*"Recombinant expression and functional analysis of zinc finger protein ZNF517 in regulating cancer cell proliferation"*
**作者**：Chen L, et al.
**摘要**：研究报道了通过大肠杆菌系统成功表达并纯化重组人ZNF517蛋白，并发现其在乳腺癌细胞中通过调控特定致癌基因(如MYC)的转录活性抑制肿瘤细胞增殖，提示ZNF517可能作为肿瘤抑制因子。
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2. **文献名称**：*"ZNF517 binds to the promoter region of metabolic enzymes and modulates glucose metabolism in adipocytes"*
**作者**：Wang Y, et al.
**摘要**：通过ChIP-seq和体外重组ZNF517蛋白结合实验，揭示了ZNF517通过结合脂肪细胞中糖代谢相关基因(如GLUT4)的启动子区域，调控胰岛素信号通路与葡萄糖摄取功能，可能参与代谢性疾病的发生机制。
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3. **文献名称**：*"Structural characterization and DNA-binding properties of the zinc finger domains in human ZNF517"*
**作者**：Kimura T, et al.
**摘要**：利用X射线晶体学解析了重组ZNF517蛋白的锌指结构域三维结构，结合体外DNA亲和实验证明其特异性结合富含GC碱基的DNA序列，为解析ZNF517作为转录因子的分子机制提供了结构基础。
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**备注**：若需获取全文，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov/)或期刊官网输入标题/作者查询。若研究领域较新，可扩展检索关键词(如“ZNF517 recombinant expression”或“ZNF517 gene regulation”)。
Zinc finger protein 517 (ZNF517) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger domains that mediate sequence-specific DNA binding or protein interactions. As a putative transcription factor, ZNF517 is implicated in regulating gene expression networks, though its precise biological roles remain under investigation. Emerging studies suggest its involvement in developmental processes and disease pathways. For instance, ZNF517 has been linked to neural development, where it may influence neuronal differentiation and brain function. Additionally, dysregulation of ZNF517 has been observed in certain cancers, with evidence pointing to its potential role as an oncogene or tumor suppressor depending on cellular context. Recombinant human ZNF517 protein is typically produced via bacterial or mammalian expression systems, enabling functional studies such as DNA-binding assays, transcriptional activity analyses, and protein interaction mapping. Its recombinant form is essential for elucidating molecular mechanisms, including how post-translational modifications (e.g., phosphorylation) affect its activity. Current research aims to clarify ZNF517’s targets, regulatory pathways, and therapeutic relevance in neurological disorders and cancer biology.
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