| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF482 |
| Uniprot No | Q15916 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424 aa |
| 活性数据 | MAAESDVLHFQFEQQGDVVLQKMNLLRQQNLFCDVSIYINDTEFQGHKVILAACSTFMRDQFLLTQSKHVRITILQSAEVGRKLLLSCYTGALEVKRKELLKYLTAASYLQMVHIVEKCTEALSKYLEIDLSMKNNNQHTDLCQSSDPDVKNEDENSDKDCEIIEISEDSPVNIDFHVKEEESNALQSTVESLTSERKEMKSPELSTVDIGFKDNEICILHVESISTAGVENGQFSQPCTSSKASMYFSETQHSLINSTVESRVAEVPGNQDQGLFCENTEGSYGTVSEIQNLEEGYSLRHQCPRCPRGFLHVENYLRHLKMHKLFLCLQCGKTFTQKKNLNRHIRGHMGIRPFQCTVCLKTFTAKSTLQDHLNIHSGDRPYKCHCCDMDFKHKSALKKHLTSVHGRSSGEKLSRPDLKRQSLL |
| 分子量 | 74.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟的参考示例(部分信息为虚构，建议通过学术数据库核实真实文献)：
1. **文献名称**: "Functional characterization of recombinant human ZNF482 as a transcriptional repressor in cancer cells"
**作者**: Liu X, et al.
**摘要**: 研究通过重组表达ZNF482蛋白，发现其通过结合特定DNA序列抑制肿瘤相关基因转录，可能参与癌细胞增殖调控。
2. **文献名称**: "Structural insights into the DNA-binding domain of ZNF482 using recombinant protein crystallography"
**作者**: Park JH, et al.
**摘要**: 解析了重组ZNF482蛋白锌指结构域的晶体结构，揭示了其与DNA相互作用的关键氨基酸位点。
3. **文献名称**: "ZNF482 regulates adipocyte differentiation through modulation of PPARγ activity"
**作者**: Chen R, et al.
**摘要**: 利用重组ZNF482蛋白实验表明，其通过调控PPARγ通路抑制脂肪细胞分化，提示代谢疾病中的潜在作用。
4. **文献名称**: "High-yield expression and purification of recombinant human ZNF482 in E. coli for functional studies"
**作者**: Gupta S, et al.
**摘要**: 开发了高效大肠杆菌重组表达系统，获得高纯度ZNF482蛋白，为后续生化研究提供可靠材料。
**注**：建议通过 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Google Scholar** 输入关键词 "ZNF482 recombinant protein" 查询真实文献。实际研究中需确保文献来源的准确性。
Zinc finger protein 482 (ZNF482), a member of the zinc finger protein family, is a transcription regulatory protein characterized by its conserved C2H2-type zinc finger domains that facilitate DNA or RNA binding. Encoded by the *ZNF482* gene in humans, it is predominantly localized to the nucleus, where it modulates gene expression through interactions with specific DNA sequences or chromatin remodeling complexes. While its precise biological functions remain under investigation, ZNF482 is hypothesized to play roles in cellular differentiation, developmental processes, and tumorigenesis. Dysregulation of ZNF proteins is frequently linked to cancers and neurological disorders, though ZNF482's exact pathological mechanisms are not fully elucidated.
Recombinant human ZNF482 protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by affinity chromatography purification for research applications. It serves as a tool to study DNA-protein interactions, gene regulation networks, and epigenetic modifications. Recent studies suggest its potential involvement in modulating cell cycle progression and apoptosis, with implications for cancer therapeutics. Structural analyses of recombinant ZNF482. including crystallography and mutagenesis, aim to decipher its DNA-binding specificity and functional domains. Ongoing research seeks to uncover its interactions with other transcriptional regulators and its role in disease pathways.
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