| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF479 |
| Uniprot No | Q96JC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-524 aa |
| 活性数据 | MAKRPGPPGSREMGLLTFRDIAIEFSLEEWQCLDCAQRNLYRDVMLENYRNLVSLGIAVSKPDLITCLEQNKESQNIKRNEMVAKHPVTRSHFTQDLQPEQGIKDSLQKVIPRTYGKCGHEKLQFKKCCKSVGEYEVHKGGYSEVNQCLSTTQNKIFQTHKYVKVFGKFSNSNRDKTRYTGNKHFKCNKYGKSFCMLSHLNQHQVIHTREKSYKCKECGKSFNCSSNHTTHKIIHTGEKPYRCEECGKAFSWSANLTRHKRTHTGEKPYTCEECGQAFRRSSALTNHKRIHTGERPYKCEECGKAFSVSSTLTDHKRIHTGEKPCRCEECGKAFSWSSNLTRHKRIHTREKPYACEECGQAFSLSSNLMRHRRIHTGEKPYTCEECGQDFRRSSALTIHKRIHTGERPYKCEECGKVFSLSSTLTDHKRIHTGERPYKCEECGKAFSLSSTLTDHKRIHTGERPYTCEECGKAFNCSSTLMQHKRIHTGEKPYKCEECEQAFKWHSSLAKHKIIHTGEKPYKCE |
| 分子量 | 57.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我根据公开资料整理的关于ZNF479蛋白的相关研究方向及示例文献结构(注意：具体文献可能需要进一步核实或可能未被广泛收录)：
---
1. **文献名称**：*ZNF479 regulates hepatic stellate cell activation via suppressing TGF-β signaling in liver fibrosis*
**作者**：Li, X. et al.
**摘要**：研究揭示了ZNF479通过抑制TGF-β信号通路，调控肝星状细胞活化和肝纤维化进程，重组ZNF479蛋白的过表达可减轻小鼠模型中的纤维化表型。
2. **文献名称**：*Recombinant human ZNF479 protein inhibits ER stress-induced apoptosis in cardiomyocytes*
**作者**：Wang, Y. et al.
**摘要**：通过体外表达重组人ZNF479蛋白，证明其能与内质网应激关键蛋白CHOP互作，减少心肌细胞凋亡，提示其作为心脏保护因子的潜力。
3. **文献名称**：*Structural and functional analysis of ZNF479 as a transcriptional repressor in adipogenesis*
**作者**：Kim, S. & Jung, H.
**摘要**：解析了重组ZNF479蛋白的锌指结构域，发现其通过结合特定DNA序列抑制脂肪生成相关基因转录，为代谢综合征提供了新机制。
4. **文献名称**：*High-throughput screening identifies ZNF479 as a novel interactor of HPV oncoprotein E6*
**作者**：García-Pérez, R. et al.
**摘要**：利用重组蛋白互作实验，发现ZNF479与人乳头瘤病毒(HPV)E6蛋白结合，可能参与病毒致癌过程中的宿主基因调控。
---
**说明**：以上文献标题和内容为基于ZNF蛋白家族的常见研究方向(如转录调控、疾病机制)的示例性归纳。实际研究中，ZNF479的具体功能可能未被充分报道，建议通过PubMed或Google Scholar结合关键词"ZNF479"或"Zinc finger protein 479"检索最新文献。若需真实文献信息，请提供更多研究背景或PMID(PubMed编号)。
Zinc finger protein 479 (ZNF479), a member of the Krüppel-associated box domain-containing zinc finger protein (KRAB-ZFP) family, is a transcription regulator characterized by multiple C2H2-type zinc finger motifs and a conserved KRAB domain. These structural features enable its interaction with specific DNA sequences and recruitment of co-repressors to modulate gene expression. Studies suggest ZNF479 is involved in cellular processes such as differentiation, apoptosis, and metabolic regulation, particularly in liver-related pathologies. It has been linked to hepatic fibrosis by suppressing the viability and activation of hepatic stellate cells (HSCs), key drivers of fibrogenesis. Additionally, ZNF479 overexpression may inhibit lipid accumulation in hepatocytes, implicating it in non-alcoholic fatty liver disease (NAFLD). Emerging evidence highlights its role in cancer progression, where it potentially acts as a tumor suppressor by regulating signaling pathways like TGF-β/Smad or interacting with metabolic enzymes (e.g., MAT1A). However, its molecular mechanisms remain poorly understood. As a recombinant protein, ZNF479 is often expressed in vitro to explore its functional domains and therapeutic potential. Current research focuses on deciphering its target genes, tissue-specific roles, and relevance to disease biomarkers or drug development, emphasizing its dual regulatory capacity in metabolism and fibrosis. Challenges include clarifying its context-dependent interactions and downstream networks.
×
