| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF46 |
| Uniprot No | P24278 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435 aa |
| 活性数据 | MDTASHSLVLLQQLNMQREFGFLCDCTVAIGDVYFKAHRAVLAAFSNYFKMIFIHQTSECIKIQPTDIQPDIFSYLLHIMYTGKGPKQIVDHSRLEEGIRFLHADYLSHIATEMNQVFSPETVQSSNLYGIQISTTQKTVVKQGLEVKEAPSSNSGNRAAVQGDHPQLQLSLAIGLDDGTADQQRACPATQALEEHQKPPVSIKQERCDPESVISQSHPSPSSEVTGPTFTENSVKIHLCHYCGERFDSRSNLRQHLHTHVSGSLPFGVPASILESNDLGEVHPLNENSEALECRRLSSFIVKENEQQPDHTNRGTTEPLQISQVSLISKDTEPVELNCNFSFSRKRKMSCTICGHKFPRKSQLLEHMYTHKGKSYRYNRCQRFGNALAQRFQPYCDSWSDVSLKSSRLSQEHLDLPCALESELTQENVDTILVE |
| 分子量 | 73.37 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为示例性参考文献，因公开数据库中针对ZNF46蛋白的直接研究较少，具体文献需根据实际检索结果调整：
1. **"ZNF46 regulates neuronal differentiation through chromatin remodeling"**
*作者：Li X, et al. (2022)*
摘要：研究利用重组人ZNF46蛋白进行体外实验，发现其通过结合特定基因启动子区域并招募组蛋白脱乙酰酶(HDACs)，调控神经干细胞分化的表观遗传机制。
2. **"Expression and purification of recombinant human ZNF46 in E. coli for structural analysis"**
*作者：Wang Y, et al. (2019)*
摘要：报道了一种高效的大肠杆菌表达系统，用于生产高纯度重组ZNF46蛋白，并通过质谱和圆二色谱验证其锌指结构域的正确折叠。
3. **"Functional screening of zinc finger proteins identifies ZNF46 as a modulator of p53 signaling"**
*作者：Chen R, et al. (2017)*
摘要：通过功能筛选发现ZNF46能与p53蛋白互作，重组ZNF46的过表达增强p53依赖的凋亡通路，提示其在肿瘤抑制中的潜在作用。
4. **"Genome-wide binding patterns of ZNF46 reveal its role in KRAB-mediated transcriptional repression"**
*作者：Garcia-Blanco M, et al. (2015)*
摘要：利用ChIP-seq分析重组ZNF46在细胞核内的DNA结合特性，发现其靶向转座子区域并参与KRAB-ZFP家族典型的基因沉默功能。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar以**"ZNF46"**或**"zinc finger protein 46"**为关键词检索。若文献不足，建议扩大检索范围至同家族蛋白(如ZNF146、ZNF384)或确认蛋白编号准确性。
**Background of Recombinant Human ZNF46 Protein**
The recombinant human Zinc Finger Protein 46 (ZNF46), also known as ZNF133. belongs to the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs. These proteins are critical for DNA binding and transcriptional regulation, often acting as repressors by recruiting chromatin-modifying complexes. ZNF46 is encoded by the *ZNF46* gene located on chromosome 19q13.2 and is implicated in regulating cellular processes such as differentiation, apoptosis, and proliferation.
Structurally, ZNF46 contains a KRAB domain at its N-terminus, which mediates protein-protein interactions, and a C-terminal array of zinc finger motifs that confer sequence-specific DNA-binding activity. Recombinant ZNF46 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling functional studies to dissect its role in gene regulatory networks.
Research highlights ZNF46's potential involvement in cancer pathogenesis, with studies suggesting its dysregulation in malignancies like gastric and colorectal cancers. Its interaction with epigenetic modifiers, such as histone deacetylases (HDACs), underscores its relevance in epigenetic silencing. Additionally, ZNF46 may contribute to neurological functions, though detailed mechanisms remain elusive.
As a research tool, recombinant ZNF46 facilitates investigations into zinc finger protein biology, chromatin remodeling, and disease pathways, offering insights for therapeutic targeting in oncology and genetic disorders.
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