| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF462 |
| Uniprot No | Q96JM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-884 aa |
| 活性数据 | MTTEVSPSQVSITEEEVGEEPVSTSHFSTSHLVSHTVFRCQLCKYFCSTRKGIARHYRIKHNNVRAQPEGKNNLFKCALCAYTNPIRKGLAAHYQKRHDIDAYYTHCLAASRTISDKPNKVIIPSPPKDDSPQLSEELRRAVEKKKCSLCSFQSFSKKGIVSHYMKRHPGVFPKKQHASKLGGYFTAVYADEHEKPTLMEEEERGSFEKAEVEGEAQEIEWLPFRCIKCFKLSFSTAELLCMHYTDHHSRDLKRDFIILGNGPRLQNSTYQCKHCDSKLQSTAELTSHLNIHNEEFQKRAKRQERRKQLLSKQKYADGAFADFKQERPFGHLEEVPKIKERKVVGYKCKFCVEVHPTLRAICNHLRKHVQYGNVPAVSAAVKGLRSHERSHLALAMFTREDKYSCQYCSFVSAFRHNLDRHMQTHHGHHKPFRCKLCSFKSSYNSRLKTHILKAHAGEHAYKCSWCSFSTMTISQLKEHSLKVHGKALTLPRPRIVSLLSSHSHHSSQKATPAEEVEDSNDSSYSEPPDVQQQLNHYQSAALARNNSRVSPVPLSGAAAGTEQKTEAVLHCEFCEFSSGYIQSIRRHYRDKHGGKKLFKCKDCSFYTGFKSAFTMHVEAGHSAVPEEGPKDLRCPLCLYHTKYKRNMIDHIVLHREERVVPIEVCRSKLSKYLQGVVFRCDKCTFTCSSDESLQQHIEKHNELKPYKCQLCYYETKHTEELDSHLRDEHKVSRNFELVGRVNLDQLEQMKEKMESSSSDDEDKEEEMNSKAEDRELMRFSDHGAALNTEKRFPCEFCGRAFSQGSEWERHVLRHGMALNDTKQVSREEIHPKEIMENSVKMPSIEEKEDDEAIGIDFSLKNETVAICVVTADKSLLENAEAKKE |
| 分子量 | 128.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人ZNF462蛋白的参考文献示例(注：文献为虚拟示例，实际引用需查询真实数据库)：
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1. **文献名称**: "Functional Characterization of Recombinant Human ZNF462 in Neural Crest Development"
**作者**: Smith A, et al.
**摘要**: 通过重组ZNF462蛋白体外实验，揭示其结合特定DNA启动子区域并调控神经嵴细胞分化基因的表达，暗示其在颅面发育中的关键作用。
2. **文献名称**: "Efficient Expression and Purification of ZNF462 in a Mammalian System for Structural Studies"
**作者**: Lee J, et al.
**摘要**: 报道了在HEK293细胞中重组表达并纯化人源ZNF462蛋白的方法，获得高纯度蛋白用于后续晶体结构解析及互作蛋白筛选。
3. **文献名称**: "Loss-of-Function Mutations in ZNF462 Disrupt Transcriptional Activity via Impaired DNA Binding"
**作者**: Garcia R, et al.
**摘要**: 结合重组ZNF462突变体蛋白实验，证明与疾病相关的错义突变导致其DNA结合能力丧失，进而影响下游靶基因(如SOX9)的调控。
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**提示**：实际研究中建议通过PubMed或Google Scholar检索关键词“ZNF462 recombinant protein”获取最新文献，并结合具体研究方向筛选。
Zinc finger protein 462 (ZNF462) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KRAB-ZFP) family, characterized by its ability to bind DNA via C-terminal C2H2-type zinc finger motifs. Predominantly expressed during embryonic development, ZNF462 is implicated in transcriptional regulation and chromatin remodeling, processes critical for cellular differentiation and tissue morphogenesis. Its N-terminal KRAB domain is thought to recruit epigenetic modifiers, such as histone methyltransferases, to mediate gene silencing at specific genomic loci. Research highlights its role in craniofacial and neural development, with ZNF462 mutations linked to rare genetic disorders like 9q31.1q31.3 microdeletion syndrome, which manifests as craniosynostosis, facial dysmorphism, and developmental delays.
Studies in model organisms, including zebrafish and mice, demonstrate that ZNF462 deficiency disrupts neural crest cell migration and patterning, underscoring its importance in embryogenesis. Additionally, ZNF462 has been associated with neurodevelopmental conditions, such as autism spectrum disorder, though its exact mechanisms remain under investigation. Recombinant human ZNF462 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), is used to study its DNA-binding specificity, interaction partners, and functional domains. This protein serves as a tool to explore its regulatory roles in development and disease, offering potential insights into therapeutic strategies for congenital and neurodevelopmental disorders.
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