| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF45 |
| Uniprot No | O43167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-682 aa |
| 活性数据 | MTKSKEAVTFKDVAVVFSEEELQLLDLAQRKLYRDVMLENFRNVVSVGHQSTPDGLPQLEREEKLWMMKMATQRDNSSGAKNLKEMETLQEVGLRYLPHEELFCSQIWQQITRELIKYQDSVVNIQRTGCQLEKRDDLHYKDEGFSNQSSHLQVHRVHTGEKPYKGEHCVKSFSWSSHLQINQRAHAGEKPYKCEKCDNAFRRFSSLQAHQRVHSRAKSYTNDASYRSFSQRSHLPHHQRVPTGENPYKYEECGRNVGKSSHCQAPLIVHTGEKPYKCEECGVGFSQRSYLQVHLKVHTGKKPYKCEECGKSFSWRSRLQAHERIHTGEKPYKCNACGKSFSYSSHLNIHCRIHTGEKPYKCEECGKGFSVGSHLQAHQISHTGEKPYKCEECGKGFCRASNLLDHQRGHTGEKPYQCDACGKGFSRSSDFNIHFRVHTGEKPYKCEECGKGFSQASNLLAHQRGHTGEKPYKCGTCGKGFSRSSDLNVHCRIHTGEKPYKCERCGKAFSQFSSLQVHQRVHTGEKPYQCAECGKGFSVGSQLQAHQRCHTGEKPYQCEECGKGFCRASNFLAHRGVHTGEKPYRCDVCGKRFRQRSYLQAHQRVHTGERPYKCEECGKVFSWSSYLQAHQRVHTGEKPYKCEECGKGFSWSSSLIIHQRVHADDEGDKDFPSSEDSHRKTR |
| 分子量 | 100.76 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设和常见锌指蛋白研究结构的参考文献示例。由于实际研究中关于“ZNF45”的文献可能有限，建议核实基因名称的正确性(如是否为ZNF451或其他相似编号)。以下是示例形式：
1. **"Cloning and Expression Analysis of Human ZNF45 in Escherichia coli"**
- 作者：Smith J, et al.
- 摘要：研究描述了ZNF45基因的克隆策略，并成功在大肠杆菌中表达重组蛋白，通过Western blot验证其表达，为后续功能研究奠定基础。
2. **"ZNF45 Regulates Apoptosis via Interaction with p53 in Cancer Cells"**
- 作者：Zhang L, et al.
- 摘要：利用重组ZNF45蛋白进行体外实验，发现其通过结合p53调控肿瘤细胞凋亡通路，提示其在癌症治疗中的潜在作用。
3. **"Structural Characterization of ZNF45 Zinc Finger Domains by NMR"**
- 作者：Lee S, et al.
- 摘要：通过核磁共振技术解析重组ZNF45蛋白的锌指结构域，揭示其与DNA结合的特定构象，为设计基因编辑工具提供参考。
4. **"ZNF45 Suppresses Viral Replication by Enhancing Innate Immune Signaling"**
- 作者：Garcia M, et al.
- 摘要：实验表明重组ZNF45蛋白可激活干扰素通路，抑制RNA病毒复制，提示其在抗病毒治疗中的应用潜力。
**注意**：以上文献为假设性示例，实际研究中可能与ZNF45相关的公开数据较少。建议通过学术数据库(如PubMed、Google Scholar)以准确基因名称检索最新文献，或验证是否存在拼写/命名错误(如ZNF451)。
Recombinant human ZNF45 protein is derived from the zinc finger protein 45 (ZNF45) gene, which encodes a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family. These proteins are characterized by tandem C2H2-type zinc finger motifs, which facilitate sequence-specific DNA binding, and a KRAB domain at the N-terminus that often mediates transcriptional repression via interactions with co-repressors. ZNF45 is implicated in regulating gene expression, particularly in processes like chromatin remodeling, cell differentiation, and apoptosis, though its precise biological roles remain less characterized compared to other KZNFs.
The recombinant form is typically produced using expression systems like Escherichia coli or mammalian cells, enabling studies of its structure and function. Its modular design—combining DNA-binding zinc fingers and effector domains—makes it a candidate for synthetic biology applications, such as engineered transcription factors. Research on ZNF45 also explores potential links to diseases, including cancer, where zinc finger proteins may act as tumor suppressors or oncogenes depending on context. However, mechanistic insights into ZNF45-specific pathways are still emerging.
Current studies focus on elucidating its target genes, interaction partners, and post-translational modifications. Recombinant ZNF45 tools aid in dissecting its role in gene networks and testing therapeutic hypotheses, bridging gaps in understanding KRAB-ZNF protein diversity and evolution.
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