| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDTC1 |
| Uniprot No | Q8N5D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677 aa |
| 活性数据 | MAKVNITRDL IRRQIKERGA LSFERRYHVT DPFIRRLGLE AELQGHSGCV NCLEWNEKGD LLASGSDDQH TIVWDPLHHK KLLSMHTGHT ANIFSVKFLP HAGDRILITG AADSKVHVHD LTVKETIHMF GDHTNRVKRI ATAPMWPNTF WSAAEDGLIR QYDLRENSKH SEVLIDLTEY CGQLVEAKCL TVNPQDNNCL AVGASGPFVR LYDIRMIHNH RKSMKQSPSA GVHTFCDRQK PLPDGAAQYY VAGHLPVKLP DYNNRLRVLV ATYVTFSPNG TELLVNMGGE QVYLFDLTYK QRPYTFLLPR KCHSSGEVQN GKMSTNGVSN GVSNGLHLHS NGFRLPESRG HVSPQVELPP YLERVKQQAN EAFACQQWTQ AIQLYSKAVQ RAPHNAMLYG NRAAAYMKRK WDGDHYDALR DCLKAISLNP CHLKAHFRLA RCLFELKYVA EALECLDDFK GKFPEQAHSS ACDALGRDIT AALFSKNDGE EKKGPGGGAP VRLRSTSRKD SISEDEMVLR ERSYDYQFRY CGHCNTTTDI KEANFFGSNA QYIVSGSDDG SFFIWEKETT NLVRVLQGDE SIVNCLQPHP SYCFLATSGI DPVVRLWNPR PESEDLTGRV VEDMEGASQA NQRRMNADPL EVMLLNMGYR ITGLSSGGAG ASDDEDSSEG QVQCRPS |
| 分子量 | 75.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDTC1蛋白的3篇参考文献的简要整理(注：文献标题及作者为虚拟示例，实际需以真实论文为准)：
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1. **文献名称**：*"Recombinant expression and purification of human WDTC1 in E. coli: implications for adipogenesis regulation"*
**作者**：Smith J. et al. (2018)
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人WDTC1蛋白，验证其与AMPK复合物的相互作用。实验表明WDTC1通过抑制脂质合成相关基因的表达调控脂肪细胞分化。
2. **文献名称**：*"WDTC1. a novel component of the CRL4 ubiquitin ligase complex, modulates lipid metabolism"*
**作者**：Li H. et al. (2016)
**摘要**：首次发现WDTC1作为CRL4泛素连接酶复合物的新成员，重组人WDTC1蛋白被用于体外泛素化实验，证明其通过促进特定底物泛素化影响肝脏脂代谢，揭示了其在肥胖中的潜在作用机制。
3. **文献名称**：*"Structural characterization of human WDTC1 by X-ray crystallography reveals a conserved WD40 domain architecture"*
**作者**：Wang Y. et al. (2020)
**摘要**：通过X射线晶体学解析重组人WDTC1蛋白的WD40结构域三维结构，发现该结构域在物种间高度保守，并参与蛋白-蛋白相互作用，为研究其代谢调控功能提供结构基础。
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**注意**：以上文献为示例，实际研究需参考真实发表的论文(建议通过PubMed或Google Scholar检索具体文献)。真实研究中，可关注WDTC1在泛素化、脂肪代谢及肥胖相关信号通路中的功能分析。
**Recombinant human WDTC1 protein** is a genetically engineered form of the WD40. TPR, and coiled-coil domain-containing protein 1. encoded by the *WDTC1* gene. WDTC1. also known as Adipose tissue triglyceride lipase (ATGL) regulator or "Adp," plays a regulatory role in lipid metabolism and energy homeostasis. Structurally, it contains WD40 repeats, tetratricopeptide (TPR) motifs, and coiled-coil domains, which mediate protein-protein interactions and cellular localization.
Biologically, WDTC1 acts as a suppressor of triglyceride hydrolysis by inhibiting ATGL activity, thereby influencing adipocyte lipid storage and systemic energy balance. Studies link it to metabolic disorders, such as obesity and diabetes, and suggest its interaction with peroxisome proliferator-activated receptor gamma (PPARγ), a master regulator of adipogenesis.
Recombinant WDTC1 is typically produced in expression systems (e.g., *E. coli*, mammalian cells*) to ensure high purity and functionality for experimental applications. It enables researchers to study its molecular mechanisms in lipid metabolism, screen for modulators in drug discovery, and explore its role in metabolic pathways. Its recombinant form is often tagged (e.g., His-tag) for purification and detection, facilitating *in vitro* assays, structural studies, and interaction analyses. Ongoing research aims to elucidate its therapeutic potential in metabolic diseases and cancer, where dysregulated lipid metabolism is implicated.
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