| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR49 |
| Uniprot No | Q8IV35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697 aa |
| 活性数据 | MAWREKSKKR LNMTSFNIAQ GIHAFDYHSR LNLIATAGIN NKVCLWNPYV VSKPVGVLWG HSASVIAVQF FVERKQLFSF SKDKVLRLWD IQHQLSIQRI ACSFPKSQDF RCLFHFDEAH GRLFISFNNQ LALLAMKSEA SKRVKSHEKA VTCVLYNSIL KQVISSDTGS TVSFWMIDTG QKIKQFTGCH GNAEISTMAL DANETRLLTG STDGTVKIWD FNGYCHHTLN VGQDGAVDIS QILILKKKIL VTGWERAITV FRPQNFNQFF IQPEEWKGGI QHHDDILCAA FLPPQTLVTG SYDGEIVLWN NSTENAHHVL HPDYQRLLKS KLDTKPQKLL SAGRSQPSHP MADHSTTGVR NFEIDTEGKN AVMRLCFLKA RKNTAVTGGA NLVSCGGSGY VRFWDIYKKQ LLAEFLAHSG VGSIIMSTDK MNRYLTTGDL DGWLKIWNIE EYCLNSSKNK ITKAPTLIRS FQPHEDRISS LEMCEPGGQL LIISSSADCS ICVTGVCNAP VWIFGQAKHW HIENCLFLPK RDTNLVESEI QKEISLFSKE ESCLDPTEHS LLNKKNKDDS TYNVRPSEDI NLDIKYKERS TCMKETQKPY YGEVIKKSFS TFRSLNIGAL EELPEVNKPA FLLDPEKYFR KEPEEERPQI LEAPSLFKTL KAVFDEKNLF PKEILHHERK AKQLCQEKSC EVKKNKK |
| 分子量 | 119.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WDR49蛋白的假设性参考文献示例(建议通过PubMed或Google Scholar检索真实文献)：
1. **"WDR49 interacts with CEP290 to regulate ciliary trafficking and signaling"**
*作者：Smith J et al.*
**摘要**：研究揭示WDR49与CEP290蛋白在纤毛运输中的相互作用，通过调控IFT复合体动态影响Hedgehog信号通路，缺失导致小鼠模型多囊肾病。
2. **"WDR49 modulates autophagosome-lysosome fusion via ATG16L1 interaction"**
*作者：Chen L et al.*
**摘要**：阐明WDR49通过结合自噬关键蛋白ATG16L1.促进自噬小体成熟，其敲除导致细胞自噬缺陷与炎症反应异常。
3. **"Genetic ablation of Wdr49 disrupts left-right asymmetry in zebrafish"**
*作者：Lee S et al.*
**摘要**：斑马鱼模型中，WDR49缺失引起纤毛结构异常，导致胚胎左右体轴发育缺陷，证实其在早期发育中的必要性。
4. **"Structural insights into WDR49’s WD40 domain and its interactome"**
*作者：Zhang Y et al.*
**摘要**：通过冷冻电镜解析WDR49的WD40结构域，预测其与分子伴侣复合物的互作网络，提示潜在分子调控机制。
*注：以上为基于研究领域推断的假设性文献，具体文献请通过学术数据库检索确认。*
WDR49 (WD repeat domain 49) is a protein encoded by the WDR49 gene in humans, belonging to the WD-repeat protein family. These proteins are characterized by conserved WD40 domains, which typically form β-propeller structures mediating protein-protein interactions. Although the full functional scope of WDR49 remains under investigation, studies suggest its involvement in critical cellular processes, particularly cilia-related functions. It has been implicated in ciliogenesis, the assembly and maintenance of motile and primary cilia, which are essential for cell signaling, fluid movement, and sensory functions.
WDR49 is highly expressed in ciliated tissues, including the respiratory tract, brain, and reproductive organs. Research links it to regulating intraflagellar transport (IFT), a process vital for cilia formation and function. Dysregulation of WDR49 may contribute to ciliopathies—genetic disorders affecting cilia—such as primary ciliary dyskinesia (PCD), infertility, or congenital respiratory defects. Mutations in WDR49 or interacting partners have been associated with disrupted ciliary motility, abnormal sperm development, and female reproductive challenges.
Emerging evidence also connects WDR49 to broader roles, including cell cycle control and DNA repair, possibly through interactions with proteins like TALPID3 and components of the cytoplasmic dynein complex. Its conserved evolutionary profile across species underscores its biological importance. Despite progress, mechanistic details of WDR49's molecular pathways and disease implications remain partially unresolved, necessitating further functional studies to explore its therapeutic potential in ciliopathies and related disorders.
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