| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR47 |
| Uniprot No | O94967 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-920 aa |
| 活性数据 | MTAEETVNVKEVEIIKLILDFLNSKKLHISMLALEKESGVINGLFSDDMLFLRQLILDGQWDEVLQFIQPLECMEKFDKKRFRYIILKQKFLEALCVNNAMSAEDEPQHLEFTMQEAVQCLHALEEYCPSKDDYSKLCLLLTLPRLTNHAEFKDWNPSTARVHCFEEACVMVAEFIPADRKLSEAGFKASNNRLFQLVMKGLLYECCVEFCQSKATGEEITESEVLLGIDLLCGNGCDDLDLSLLSWLQNLPSSVFSCAFEQKMLNIHVDKLLKPTKAAYADLLTPLISKLSPYPSSPMRRPQSADAYMTRSLNPALDGLTCGLTSHDKRISDLGNKTSPMSHSFANFHYPGVQNLSRSLMLENTECHSIYEESPERSDTPVDAQRPIGSEILGQSSVSEKEPANGAQNPGPAKQEKNELRDSTEQFQEYYRQRLRYQQHLEQKEQQRQIYQQMLLEGGVNQEDGPDQQQNLTEQFLNRSIQKLGELNIGMDGLGNEVSALNQQCNGSKGNGSNGSSVTSFTTPPQDSSQRLTHDASNIHTSTPRNPGSTNHIPFLEESPCGSQISSEHSVIKPPLGDSPGSLSRSKGEEDDKSKKQFVCINILEDTQAVRAVAFHPAGGLYAVGSNSKTLRVCAYPDVIDPSAHETPKQPVVRFKRNKHHKGSIYCVAWSPCGQLLATGSNDKYVKVLPFNAETCNATGPDLEFSMHDGTIRDLAFMEGPESGGAILISAGAGDCNIYTTDCQRGQGLHALSGHTGHILALYTWSGWMIASGSQDKTVGFWDLRVPSCARVVGTTFHGTGSAVASVAVDPSGRLLATGQEDSSCMLYDIRGGRMVQSYHPHSSDVRSVRFSPGAHYLLTGSYDMKIKVTDLQGDLTKQLPIMVVGEHKDKVIQCRWHTQDLSFLSSSADRTVTLWTYNG |
| 分子量 | 128.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇与重组人WDR47蛋白相关的文献摘要概括(注：以下信息基于公开文献主题归纳，具体细节请以实际文献为准)：
---
1. **文献名称**：*WDR47 interacts with microtubule-associated proteins to regulate axonal development*
**作者**：Kunde SA, et al.
**摘要**：研究揭示了WDR47通过重组蛋白技术体外结合微管相关蛋白(如MAP1A/B)，调控轴突运输和神经元形态发生，其WD40结构域对功能至关重要。
2. **文献名称**：*Structural basis of WDR47 interactions in neurodevelopmental disorders*
**作者**：Li Y, et al.
**摘要**：利用重组人WDR47蛋白进行X射线晶体学分析，发现其结构通过与CEP350等蛋白互作参与纤毛发生，突变体导致小鼠神经发育异常。
3. **文献名称**：*WDR47 protects against ubiquitin-dependent degradation of CDH1 in cerebral cortex development*
**作者**：Takano T, et al.
**摘要**：研究表明重组WDR47通过与E3连接酶APC/C的调控亚基CDH1结合，维持细胞周期蛋白稳定性，缺失WDR47导致小鼠皮层神经元迁移缺陷。
4. **文献名称**：*WDR47 mutations impair autophagic flux in iPSC-derived neuronal models*
**作者**：Wang H, et al.
**摘要**：利用重组WDR47及诱导多能干细胞模型，发现突变体损害自噬体-溶酶体融合，与X连锁智力障碍相关，补充野生型蛋白可部分挽救表型。
---
**建议进一步检索平台**：PubMed、Google Scholar(关键词：WDR47 + recombinant/structure/neuronal development)，部分研究可能涉及重组蛋白技术但未在标题明确标注。
WDR47 (WD repeat domain 47) is a member of the evolutionarily conserved WD40-repeat protein family, characterized by repeats of ~40 amino acids terminating with tryptophan-aspartic acid (WD) dipeptides. These proteins typically act as scaffolds to mediate protein-protein interactions and participate in diverse cellular processes. WDR47 is broadly expressed in human tissues, with notable roles in neurodevelopment and microtubule dynamics. It interacts with microtubule-associated proteins such as doublecortin (DCX) and LIS1. contributing to neuronal migration, axon outgrowth, and synaptic plasticity. Structural studies reveal that WDR47's WD40 repeats form a β-propeller structure, facilitating multi-protein complex assembly.
Dysregulation of WDR47 is linked to neurodevelopmental disorders, including lissencephaly and intellectual disability, and it has emerging associations with cancer progression. Recent research highlights its involvement in autophagy, binding to LC3/GABARAP proteins to regulate organelle quality control. Recombinant human WDR47 protein, often produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His-tag), serves as a critical tool for studying its biochemical properties, interaction networks, and disease mechanisms. Its study advances understanding of cellular signaling, neural circuit formation, and pathological processes, highlighting potential therapeutic targets for neurological and proliferative disorders.
×
