| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR46 |
| Uniprot No | O15213 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-610 aa |
| 活性数据 | METAPKPGKD VPPKKDKLQT KRKKPRRYWE EETVPTTAGA SPGPPRNKKN RELRPQRPKN AYILKKSRIS KKPQVPKKPR EWKNPESQRG LSGTQDPFPG PAPVPVEVVQ KFCRIDKSRK LPHSKAKTRS RLEVAEAEEE ETSIKAARSE LLLAEEPGFL EGEDGEDTAK ICQADIVEAV DIASAAKHFD LNLRQFGPYR LNYSRTGRHL AFGGRRGHVA ALDWVTKKLM CEINVMEAVR DIRFLHSEAL LAVAQNRWLH IYDNQGIELH CIRRCDRVTR LEFLPFHFLL ATASETGFLT YLDVSVGKIV AALNARAGRL DVMSQNPYNA VIHLGHSNGT VSLWSPAMKE PLAKILCHRG GVRAVAVDST GTYMATSGLD HQLKIFDLRG TYQPLSTRTL PHGAGHLAFS QRGLLVAGMG DVVNIWAGQG KASPPSLEQP YLTHRLSGPV HGLQFCPFED VLGVGHTGGI TSMLVPGAGE PNFDGLESNP YRSRKQRQEW EVKALLEKVP AELICLDPRA LAEVDVISLE QGKKEQIERL GYDPQAKAPF QPKPKQKGRS STASLVKRKR KVMDEEHRDK VRQSLQQQHH KEAKAKPTGA RPSALDRFVR |
| 分子量 | 68.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR46蛋白的3篇参考文献示例(内容基于公开研究趋势的合理推导，非真实文献)：
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1. **文献名称**：*Structural Insights into the WD40 Domain of Human WDR46 and Its Role in mRNA Processing*
**作者**：Yamamoto, K. et al.
**摘要**：本研究解析了重组人WDR46蛋白中WD40结构域的晶体结构，揭示了其与RNA结合蛋白的相互作用模式，支持其在mRNA剪切和运输中的潜在功能。
2. **文献名称**：*WDR46 Interacts with Deubiquitinase USP15 to Modulate p53 Signaling Pathways*
**作者**：Chen, L. & Wei, R.
**摘要**：通过重组WDR46蛋白的体外实验，发现其通过结合USP15调控p53的泛素化水平，提示WDR46可能参与肿瘤发生中的细胞应激反应通路。
3. **文献名称**：*Proteomic Analysis of WDR46-Containing Complexes in Human Cell Nuclei*
**作者**：Gomez, S. et al.
**摘要**：利用重组WDR46蛋白进行免疫共沉淀-质谱分析，鉴定出多个核内相互作用蛋白(如核转运蛋白KPNB1)，表明其在核质运输和染色质重塑中的作用。
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注：以上文献为示例性内容，实际研究请通过PubMed或Google Scholar检索关键词“WDR46”或“C6orf11”获取最新数据。
WDR46 (WD repeat domain 46), also known as UTP7 or BKIB, is a conserved eukaryotic protein belonging to the WD40 repeat family. Characterized by its β-propeller structure formed by WD40 repeats, it serves as a versatile interaction platform for multiprotein complexes. WDR46 is encoded by a gene located on the X chromosome (Xq22.1 in humans) and ubiquitously expressed across tissues, suggesting fundamental roles in cellular processes.
Functionally, WDR46 is implicated in ribosome biogenesis through its association with small subunit processome components, facilitating rRNA processing. It also interacts with transcriptional regulators like PAXIP1/PTIP, linking it to histone modification and DNA damage response pathways. Studies indicate involvement in cell cycle progression, apoptosis, and stem cell differentiation, though precise mechanisms remain under investigation.
Disease associations include X-linked intellectual disability (XLID), where WDR46 mutations correlate with neuronal development defects, potentially due to disrupted ribosome or epigenetic pathways. Additionally, aberrant expression has been observed in cancers, though its oncogenic or tumor-suppressive role is context-dependent. Recombinant human WDR46 protein is utilized to study its molecular interactions, structural dynamics, and therapeutic potential. Current research focuses on delineating its role in cellular homeostasis and pathology, leveraging CRISPR models and interactome analyses to uncover mechanistic insights.
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