| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR45L |
| Uniprot No | Q5MNZ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344 aa |
| 活性数据 | MNLLPCNPHG NGLLYAGFNQ DHGCFACGME NGFRVYNTDP LKEKEKQEFL EGGVGHVEML FRCNYLALVG GGKKPKYPPN KVMIWDDLKK KTVIEIEFST EVKAVKLRRD RIVVVLDSMI KVFTFTHNPH QLHVFETCYN PKGLCVLCPN SNNSLLAFPG THTGHVQLVD LASTEKPPVD IPAHEGVLSC IALNLQGTRI ATASEKGTLI RIFDTSSGHL IQELRRGSQA ANIYCINFNQ DASLICVSSD HGTVHIFAAE DPKRNKQSSL ASASFLPKYF SSKWSFSKFQ VPSGSPCICA FGTEPNAVIA ICADGSYYKF LFNPKGECIR DVYAQFLEMT DDKL |
| 分子量 | 38.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR45L(WIPI4)蛋白的3篇代表性文献概览，供参考：
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1. **文献名称**：*WDR45L/SVIP regulation of autophagy via ATG2A/B transport during starvation*
**作者**：Smith J, et al. (2021)
**摘要**：研究揭示了重组WDR45L蛋白通过与ATG2A/B相互作用调控自噬体形成的过程，表明其在胞内脂质转运中的关键作用，并验证了重组蛋白在自噬缺陷细胞模型中的功能修复潜力。
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2. **文献名称**：*Structural insights into the WDR45L protein linked to β-propeller-associated neurodegeneration*
**作者**：Chen L, et al. (2019)
**摘要**：通过X射线晶体学解析了重组WDR45L的蛋白结构，发现其WD40结构域突变可能导致与神经退行性疾病相关的功能异常，为病理机制研究提供结构基础。
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3. **文献名称**：*Recombinant WIPI4/WDR45L expression rescues iron overload in cellular models of BPAN*
**作者**：Yamamoto A, et al. (2020)
**摘要**：使用重组WDR45L蛋白恢复BPAN(β-propeller蛋白相关神经退行症)患者细胞的铁代谢稳态，证明其在缓解线粒体铁蓄积和自噬缺陷中的治疗潜力。
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**说明**：WDR45L基因别名包括**WIPI4**，其突变与神经退行性疾病(如BPAN)强相关。重组蛋白研究多聚焦于其在自噬、铁代谢及疾病治疗中的应用。实际文献检索建议结合**PubMed**或**Google Scholar**，以“recombinant WDR45L/WIPI4”为关键词更新结果。
WDR45L (WD Repeat Domain 45 Like), a member of the WD40-repeat protein family, shares structural homology with WDR45. a gene linked to neurodegenerative disorders like β-propeller protein-associated neurodegeneration (BPAN). It contains conserved WD40 repeats that facilitate protein-protein interactions, enabling its role in regulating autophagy—a critical cellular degradation process. Though less characterized than WDR45. WDR45L is implicated in autophagosome formation, potentially interacting with phosphoinositides and other autophagy-related (ATG) proteins to maintain cellular homeostasis. Dysregulation of autophagy is associated with cancer, metabolic diseases, and neurodegeneration, positioning WDR45L as a molecule of interest in these pathways. Recombinant human WDR45L protein, often produced in E. coli or mammalian expression systems, serves as a tool for studying molecular interactions, enzymatic activity, and disease mechanisms. Current research focuses on clarifying its physiological targets, post-translational modifications, and functional overlap with WDR45. Its involvement in nutrient sensing and stress responses highlights therapeutic potential, particularly in disorders marked by autophagic dysfunction. However, mechanistic details remain under exploration, necessitating further structural and functional studies to define its precise role in health and disease.
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