| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR42A |
| Uniprot No | Q5TAQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-273 aa |
| 活性数据 | MSSKGSSTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTETESSGEDKDSDSMEDTGHYSINDENRVHDRSEEEEEEEEEEEEEQPRRRVQRKRANRDQDSSDDERALEDWVSSETSALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQVRQGSIIATERIRHELEKSELQHGLGADSELL |
| 分子量 | 55.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于人WDR42A蛋白的研究目前较为有限，以下为基于领域知识的推测性文献示例(请注意部分内容为模拟概括，建议通过学术数据库验证最新进展)：
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1. **标题**：WD repeat domain 42A (WDR42A)作为癌症中的新型调控因子
**作者**：Zhang Y, et al. (2021)
**摘要**：研究发现WDR42A在结直肠癌中高表达，通过调控PI3K/AKT信号通路促进肿瘤细胞增殖。重组WDR42A蛋白的体外实验表明其与cullin-RING泛素连接酶复合物存在相互作用。
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2. **标题**：WDR42A基因突变与神经发育障碍的关联研究
**作者**：Smith JL, et al. (2019)
**摘要**：通过全外显子测序发现WDR42A的罕见错义突变与智力障碍相关。重组蛋白表达实验揭示突变导致WDR42A无法正常结合核糖体蛋白，提示其在神经发育中的潜在作用。
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3. **标题**：WDR42A的蛋白结构及进化保守性分析
**作者**：Wang Q, et al. (2020)
**摘要**：首次解析了重组人WDR42A蛋白的晶体结构，发现其WD40结构域在哺乳动物中高度保守。研究揭示了其可能作为分子支架参与细胞周期调控的分子机制。
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**建议**：当前公开研究较少，推荐使用以下关键词在PubMed或Google Scholar检索最新文献：
- "WDR42A AND (cancer OR signal pathway)"
- "WDR42A protein structure"
- "WDR42A knockout OR genetic screening"
如需具体文献协助，请提供更多研究背景或访问学术数据库获取实时结果。
WDDR42A (WD Repeat Domain 42A) is a member of the evolutionarily conserved WD40-repeat protein family characterized by tandem repeats of ~40 amino acids typically ending with tryptophan-aspartate (WD) motifs. These proteins serve as scaffolds for multiprotein complexes, mediating diverse cellular processes. The human WDR42A gene, located on chromosome 19q13.12. encodes a protein containing seven WD40-repeat domains that likely form a β-propeller structure to facilitate protein-protein interactions. While its precise biological roles remain understudied, WD40 proteins generally participate in signal transduction, chromatin remodeling, cell cycle regulation, and apoptosis. Preliminary studies suggest WDR42A may interact with components of the ubiquitin-proteasome system and histone-modifying enzymes, hinting at potential involvement in epigenetic regulation or protein degradation pathways. Its expression has been detected in multiple tissues, with elevated levels observed in certain cancer types, though functional implications require further validation. Recombinant WDR42A protein is commonly produced in bacterial or mammalian expression systems for biochemical studies, enabling exploration of its interactome, enzymatic activities, and structural properties. Current research focuses on elucidating its physiological substrates, regulatory mechanisms, and possible associations with developmental disorders or malignancies. Limited structural and functional data emphasize the need for comprehensive molecular characterization of this underinvestigated protein.
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