| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR36 |
| Uniprot No | Q8NI36 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-895aa |
| 活性数据 | MERASERRTASALFAGFRALGLFSNDIPHVVRFSALKRRFYVTTCVGKSFHTYDVQKLSLVAVSNSVPQDICCMAADGRLVFAAYGNVFSAFARNKEIVHTFKGHKAEIHFLQPFGDHIISVDTDGILIIWHIYSEEEYLQLTFDKSVFKISAILHPSTYLNKILLGSEQGSLQLWNVKSNKLLYTFPGWKVGVTALQQAPAVDVVAIGLMSGQVIIHNIKFNETLMKFRQDWGPITSISFRTDGHPVMAAGSPCGHIGLWDLEDKKLINQMRNAHSTAIAGLTFLHREPLLVTNGADNALRIWIFDGPTGEGRLLRFRMGHSAPLTNIRYYGQNGQQILSASQDGTLQSFSTVHEKFNKSLGHGLINKKRVKRKGLQNTMSVRLPPITKFAAEEARESDWDGIIACHQGKLSCSTWNYQKSTIGAYFLKPKELKKDDITATAVDITSCGNFAVIGLSSGTVDVYNMQSGIHRGSFGKDQAHKGSVRGVAVDGLNQLTVTTGSEGLLKFWNFKNKILIHSVSLSSSPNIMLLHRDSGILGLALDDFSISVLDIETRKIVREFSGHQGQINDMAFSPDGRWLISAAMDCSIRTWDLPSGCLIDCFLLDSAPLNVSMSPTGDFLATSHVDHLGIYLWSNISLYSVVSLRPLPADYVPSIVMLPGTCQTQDVEVSEETVEPSDELIEYDSPEQLNEQLVTLSLLPESRWKNLLNLDVIKKKNKPKEPPKVPKSAPFFIPTIPGLVPRYAAPEQNNDPQQSKVVNLGVLAQKSDFCLKLEEGLVNNKYDTALNLLKESGPSGIETELRSLSPDCGGSIEVMQSFLKMIGMMLDRKRDFELAQAYLALFLKLHLKMLPSEPVLLEEITNLSSQVEENWTHLQSLFNQSMCILNYLKSALL |
| 分子量 | 99,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR36蛋白的3篇参考文献，简明摘要其研究内容：
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1. **"Mutations in the WDR36 gene associated with cellular stress and apoptosis in glaucoma"**
*作者：Skarie JM, Link BA (2008)*
**摘要**：研究通过重组WDR36蛋白实验，发现其与p53通路相互作用，调节细胞凋亡，揭示了其在青光眼病理中可能的调控机制。
2. **"Expression and functional analysis of WDR36 mutations in primary open-angle glaucoma"**
*作者：Fingert JH et al. (2009)*
**摘要**：利用重组WDR36蛋白模型，验证突变体导致应激条件下细胞存活率下降，表明其功能障碍可能参与青光眼发展。
3. **"The WD40-repeat protein WDR36 interacts with HSP70 to regulate protein homeostasis"**
*作者：Li X et al. (2015)*
**摘要**：通过体外重组蛋白实验，证明WDR36与热休克蛋白HSP70结合，协助错误折叠蛋白的清除，提示其在维持细胞稳态中的作用。
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注：若需更多文献，可检索PubMed等数据库，关键词"recombinant WDR36 protein"或"WDR36 functional study"。
WDR36 (WD40-repeat protein 36) is a member of the evolutionarily conserved WD40-repeat protein family, characterized by tandem repeats of ~40 amino acids that fold into β-propeller structures. These proteins typically serve as scaffolds for protein-protein or protein-nucleic acid interactions, regulating diverse cellular processes. Human WDR36. located on chromosome 5q22.1. encodes a 951-amino acid protein predominantly expressed in ocular tissues, the liver, and kidneys. It localizes to the nucleolus and cytoplasm, implicating roles in ribosomal RNA (rRNA) processing, cell cycle regulation, and apoptosis.
WDR36 gained attention due to its association with primary open-angle glaucoma (POAG), a leading cause of irreversible blindness. Genetic studies identified mutations in WDR36 linked to POAG pathogenesis, though its exact mechanism remains unclear. Hypotheses include dysregulation of stress-response pathways, impaired ribosomal biogenesis, or interactions with TGF-β signaling components. Recombinant human WDR36 protein, often produced via bacterial or mammalian expression systems, enables functional studies, antibody development, and drug screening. Its structural complexity and post-translational modifications pose challenges for purification, limiting its application in high-throughput assays. Current research focuses on elucidating its molecular interactome and validating its role in ocular homeostasis, offering potential therapeutic targets for glaucoma.
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