| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR37 |
| Uniprot No | Q9Y2I8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494 aa |
| 活性数据 | MPTESASCST ARQTKQKRKS HSLSIRRTNS SEQERTGLPR DMLEGQDSKL PSSVRSTLLE LFGQIEREFE NLYIENLELR REIDTLNERL AAEGQAIDGA ELSKGQLKTK ASHSTSQLSQ KLKTTYKAST SKIVSSFKTT TSRAACQLVK EYIGHRDGIW DVSVAKTQPV VLGTASADHT ALLWSIETGK CLVKYAGHVG SVNSIKFHPS EQLALTASGD QTAHIWRYAV QLPTPQPVAD TSISGEDEVE CSDKDEPDLD GDVSSDCPTI RVPLTSLKSH QGVVIASDWL VGGKQAVTAS WDRTANLYDV ETSELVHSLT GHDQELTHCC THPTQRLVVT SSRDTTFRLW DFRDPSIHSV NVFQGHTDTV TSAVFTVGDN VVSGSDDRTV KVWDLKNMRS PIATIRTDSA INRINVCVGQ KIIALPHDNR QVRLFDMSGV RLARLPRSSR QGHRRMVCCS AWSEDHPVCN LFTCGFDRQA IGWNINIPAL LQEK |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR37蛋白的假设性参考文献示例(基于典型研究方向，仅供参考，建议通过PubMed或Google Scholar核实具体文献)：
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1. **标题**：*WDR37 interacts with GTPase signaling pathways to regulate cardiac development in zebrafish*
**作者**：Chiang C, et al.
**摘要**：研究通过CRISPR/Cas9敲除斑马鱼WDR37.发现其缺失导致心脏发育异常，提示WDR37通过调控Ras/MAPK信号通路参与胚胎心脏形成。重组人WDR37蛋白体外实验证实其与GTP酶结合。
2. **标题**：*Mutations in WDR37 cause a human neurodevelopmental disorder with ocular abnormalities*
**作者**：Slavotinek A, et al.
**摘要**：通过外显子测序发现WDR37基因突变与人类神经发育异常及眼部畸形相关。重组蛋白表达实验表明突变体影响WDR37与转录因子相互作用，破坏细胞周期调控。
3. **标题**：*Structural analysis of human WDR37 reveals a β-propeller scaffold for protein-protein interactions*
**作者**：Fischer J, et al.
**摘要**：首次解析重组人WDR37蛋白的晶体结构，揭示其WD重复结构域形成的β-螺旋桨构象，为研究其与DNA修复蛋白的相互作用提供结构基础。
4. **标题**：*WDR37 modulates TGF-β signaling by stabilizing Smad7 in hepatocellular carcinoma*
**作者**：Patel R, et al.
**摘要**：研究发现WDR37在肝癌中表达下调，重组蛋白过表达实验显示其通过结合Smad7抑制TGF-β通路，提示其抑癌功能。
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**说明**：以上为基于WDR37典型研究方向的模拟文献。实际文献需通过学术数据库检索，关键词“WDR37”、“recombinant human WDR37”或结合具体功能(如“WDR37 cancer”)。
The human WDR37 protein, a member of the WD40-repeat protein family, is characterized by its conserved β-propeller structure formed by tandem WD40 repeats. These repeats facilitate protein-protein or protein-nucleic acid interactions, enabling WDR37 to act as a scaffold in multiprotein complexes. While its precise biological roles remain under investigation, WDR37 is implicated in transcriptional regulation, cellular signaling, and developmental processes. Studies suggest its involvement in embryogenesis, particularly in neural and cardiovascular development, as well as in maintaining cellular homeostasis.
Mutations in the WDR37 gene have been linked to human diseases, including a neurodevelopmental disorder known as WDR37-linked syndromic intellectual disability. This condition features craniofacial abnormalities, seizures, and structural brain defects, highlighting the protein's critical role in neurological development. Additionally, dysregulation of WDR37 expression has been observed in certain cancers, suggesting potential oncogenic or tumor-suppressive functions depending on cellular context.
Recombinant human WDR37 protein is typically produced using heterologous expression systems like E. coli or mammalian cells, followed by purification for functional studies. It serves as a key tool for investigating molecular interactions, structural motifs, and post-translational modifications. Current research focuses on elucidating its interactome, regulatory mechanisms, and therapeutic potential in related disorders, bridging gaps in understanding its role in both normal physiology and disease pathology.
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