| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR41 |
| Uniprot No | Q9HAD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459 aa |
| 活性数据 | MLRWLIGGGR EPQGLAEKSP LQTIGEEQTQ NPYTELLVLK AHHDIVRFLV QLDDYRFASA GDDGIVVVWN AQTGEKLLEL NGHTQKITAI ITFPSLESCE EKNQLILTAS ADRTVIVWDG DTTRQVQRIS CFQSTVKCLT VLQRLDVWLS GGNDLCVWNR KLDLLCKTSH LSDTGISALV EIPKNCVVAA VGKELIIFRL VAPTEGSLEW DILEVKRLLD HQDNILSLIN VNDLSFVTGS HVGELIIWDA LDWTMQAYER NFWDPSPQLD TQQEIKLCQK SNDISIHHFT CDEENVFAAV GRGLYVYSLQ MKRVIACQKT AHDSNVLHVA RLPNRQLISC SEDGSVRIWE LREKQQLAAE PVPTGFFNMW GFGRVSKQAS QPVKKQQENA TSCSLELIGD LIGHSSSVEM FLYFEDHGLV TCSADHLIIL WKNGERESGL RSLRLFQKLE ENGDLYLAV |
| 分子量 | 51.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"WDR41 supports lysosomal enzyme delivery by mediating fusion of transport carriers with late endosomes/lysosomes"**
- **作者**: Sawa-Makarska J, et al.
- **摘要**: 本研究揭示了WDR41在自噬体成熟中的关键作用，通过调控运输载体与晚期内体/溶酶体的膜融合，促进溶酶体酶递送。实验表明重组人WDR41蛋白与UBE2W、ATG5等蛋白互作，影响自噬相关泛素化通路。
2. **"WDR41 mutations impair Parkin-mediated mitophagy and cause early-onset Parkinson’s disease"**
- **作者**: Chen Z, et al.
- **摘要**: 通过全外显子测序发现WDR41的罕见突变与早发性帕金森病相关。功能实验显示，重组WDR41蛋白突变体破坏Parkin依赖性线粒体自噬，导致异常线粒体积累和神经元死亡。
3. **"WDR41 regulates GTPase-mediated vesicle trafficking via its interaction with Rab11"**
- **作者**: Hara T, et al.
- **摘要**: 报道WDR41通过结合Rab11调控细胞内膜运输，尤其是轴突内囊泡运输。重组人WDR41蛋白的体外结合实验证明其WD40结构域对Rab11相互作用至关重要，缺失导致神经元突触运输障碍。
4. **"Structural insights into the role of WDR41 in coordinating non-canonical autophagy pathways"**
- **作者**: Gholkar AA, et al.
- **摘要**: 通过冷冻电镜解析人WDR41蛋白结构，揭示其作为支架蛋白协调非经典自噬信号复合体的组装。研究强调其N端结构域在招募泛素化底物中的功能，重组蛋白被用于验证互作界面。
(注：以上文献信息为虚构示例，实际引用时需核对真实发表内容及作者信息。)
**Background of Human WD Repeat Domain 41 (WDR41) Protein**
WDR41 is a member of the WD40 repeat protein family, characterized by conserved repeats of ~40 amino acids that form β-propeller structures to mediate protein-protein interactions. It is encoded by the *WDR41* gene located on human chromosome 5 (5q31.1) and is ubiquitously expressed across tissues, with higher levels in the brain.
Functionally, WDR41 is implicated in regulating intracellular trafficking and autophagy-lysosomal pathways. Studies suggest it interacts with proteins involved in vesicle transport, such as the BEACH domain-containing protein LYST, and modulates mTOR signaling, a critical pathway for cellular homeostasis. WDR41’s role in autophagy highlights its potential link to neurodegenerative diseases, where lysosomal dysfunction is a hallmark.
Notably, WDR41 mutations or dysregulation have been tentatively associated with neurodevelopmental disorders and cancer, though mechanistic insights remain limited. Its recombinant form (recombinant WDR41) is often produced via bacterial or mammalian expression systems for structural and functional studies. Researchers utilize it to explore binding partners, post-translational modifications, and its impact on cellular pathways.
Current research focuses on elucidating WDR41’s precise molecular mechanisms and its therapeutic potential in diseases linked to autophagy or trafficking defects. Despite progress, its full biological significance and disease relevance require further investigation.
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