| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR34 |
| Uniprot No | Q96EX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536 aa |
| 活性数据 | MATRAQPGPL SQAGSAGVAA LATVGVASGP GPGRPGPLQD ETLGVASVPS QWRAVQGIRW ETKSCQTASI ATASASAQAR NHVDAQVQTE APVPVSVQPP SQYDIPRLAA FLRRVEAMVI RELNKNWQSH AFDGFEVNWT EQQQMVSCLY TLGYPPAQAQ GLHVTSISWN STGSVVACAY GRLDHGDWST LKSFVCAWNL DRRDLRPQQP SAVVEVPSAV LCLAFHPTQP SHVAGGLYSG EVLVWDLSRL EDPLLWRTGL TDDTHTDPVS QVVWLPEPGH SHRFQVLSVA TDGKVLLWQG IGVGQLQLTE GFALVMQQLP RSTKLKKHPR GETEVGATAV AFSSFDPRLF ILGTEGGFPL KCSLAAGEAA LTRMPSSVPL RAPAQFTFSP HGGPIYSVSC SPFHRNLFLS AGTDGHVHLY SMLQAPPLTS LQLSLKYLFA VRWSPVRPLV FAAASGKGDV QLFDLQKSSQ KPTVLIKQTQ DESPVYCLEF NSQQTQLLAA GDAQGTVKVW QLSTEFTEQG PREAEDLDCL AAEVAA |
| 分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人WDR34蛋白的3篇代表性文献示例(供参考，需核实实际文献存在性)：
---
1. **文献名称**: *WDR34 regulates ciliary dynein assembly and intraflagellar transport in vertebrate cells*
**作者**: Smith J et al.
**摘要**: 研究发现WDR34与动力蛋白复合体(dynein)相互作用，参与纤毛发生及轴丝运输。通过敲除实验表明，WDR34缺失导致纤毛运动障碍，提示其可能参与纤毛相关疾病的分子机制。
---
2. **文献名称**: *Structural and functional analysis of the WDR34 protein in the context of cancer progression*
**作者**: Li X et al.
**摘要**: 该文献解析了重组表达的WDR34蛋白结构，揭示其WD40结构域介导的蛋白互作网络。研究还发现WDR34在某些癌症中过表达，可能通过调控细胞周期信号通路促进肿瘤生长。
---
3. **文献名称**: *Mutations in WDR34 cause skeletal ciliopathies through disrupted Hedgehog signaling*
**作者**: Garcia-Gonzalo FR et al.
**摘要**: 通过临床样本分析，发现WDR34突变与骨骼发育异常相关。实验表明，WDR34通过调节Hedgehog信号通路中的IFT-A复合体功能，影响软骨细胞分化和骨骼形成。
---
**备注**：以上为根据WDR34已知功能构造的示例，实际文献需通过数据库(如PubMed)检索确认。建议使用关键词“WDR34 protein”或“WDR34 function”查找最新研究。
WDR34 (WD repeat-containing protein 34) is a member of the evolutionarily conserved WD40 repeat protein family, characterized by tandem repeats of ~40-60 amino acids terminating with tryptophan-aspartic acid (WD) motifs. These proteins typically act as scaffolding platforms to mediate multi-protein complex assembly. In humans, WDR34 is linked to cytoplasmic dynein-2. a motor complex critical for retrograde intraflagellar transport (IFT) within primary cilia. Cilia are microtubule-based organelles essential for signal transduction, cell motility, and developmental patterning.
Mutations in WDR34 are associated with ciliopathies such as Jeune asphyxiating thoracic dystrophy (JATD) and Sensenbrenner syndrome, disorders marked by skeletal abnormalities, respiratory defects, and renal dysfunction. The protein’s WD40 domains likely facilitate interactions with dynein-2 subunits and regulatory proteins, ensuring proper ciliary assembly and maintenance. Studies suggest WDR34 collaborates with WDR60 to form a dynein-2 intermediate chain subcomplex, influencing motor stability and cargo binding.
Recombinant human WDR34 protein is engineered for biochemical studies to dissect its structural roles in dynein-2 function and cilia-related pathologies. Its expression in heterologous systems (e.g., E. coli, mammalian cells) enables analysis of mutant variants, interactions, and mechanisms underlying ciliopathy phenotypes. Research on WDR34 enhances understanding of ciliary transport dynamics and may inform therapeutic strategies for genetic disorders tied to IFT defects.
×
