| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR27 |
| Uniprot No | A2RRH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-266 aa |
| 活性数据 | MENPQDIFSSNGGCLSDIVIEKYLVESKESVSHVQLACSMQDCAFPLDGTELCIWNTKDPSHQLLILRGHHQPITAMAFGNKVNPLLICSASLDYVIMWNLDECREKVLQGLVPRGTVMGSLLGKVLCLQLSPDDHVVAVCAGNKIFMLDIEQRFSVTYIERPDVNNRHKVPPPTFLHTFSQAQAVRAELQGHLGPVTAVEFCPWRAGTLISASEDRGFKVWDHCTGSLIYSSSVLSAYPLLSLFIDAESRQLVTGVLTASFGSSV |
| 分子量 | 55.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR27蛋白的参考文献列表(基于现有研究趋势的示例性概括，部分为假设性内容)：
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1. **标题**：*Structural and functional characterization of human WDR27 reveals a conserved role in ciliary protein trafficking*
**作者**：Chen L, et al.
**摘要**：本研究解析了WDR27蛋白的晶体结构，发现其通过WD40重复结构域介导与其他纤毛相关蛋白的相互作用，并证实其在纤毛形成和信号转导中的关键作用。
2. **标题**：*WDR27 interacts with PI3K/AKT/mTOR signaling pathway to promote tumor progression in glioblastoma*
**作者**：Wang Y, et al.
**摘要**：通过重组WDR27蛋白的功能实验，揭示其通过调控PI3K/AKT通路促进胶质母细胞瘤的增殖和侵袭，提示其作为潜在治疗靶点。
3. **标题**：*A novel WDR27 variant linked to autosomal recessive intellectual disability: Insights from recombinant protein analysis*
**作者**：Smith JL, et al.
**摘要**：在智力障碍患者中发现WDR27基因突变，重组蛋白表达显示突变体丧失与神经发育关键蛋白的结合能力，表明其功能异常导致神经发育缺陷。
4. **标题**：*Recombinant WDR27 modulates the assembly of the COP9 signalosome complex in vitro*
**作者**：Gomez-Reyes F, et al.
**摘要**：体外实验表明，重组WDR27通过结合COP9复合体亚基调控泛素-蛋白酶体系统，影响细胞内蛋白质稳态。
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**说明**：以上文献为基于WDR27相关研究领域的典型方向生成的示例性内容，实际文献需通过PubMed/Google Scholar等平台检索确认。如需具体已发表论文，建议使用关键词“WDR27 + recombinant”或联系学术数据库进一步筛选。
**Background of Recombinant Human WDR27 Protein**
The WD40 repeat domain 27 (WDR27) protein is a member of the WD40 repeat protein family, characterized by tandem repeats of ~40 amino acids often terminating with a tryptophan-aspartic acid (WD) motif. These proteins typically form β-propeller structures that facilitate protein-protein interactions, acting as scaffolds in cellular processes. While many WD40 proteins are implicated in signaling, transcription, and cell cycle regulation, the specific biological roles of WDR27 remain poorly understood.
Recombinant human WDR27 refers to the engineered version of the protein produced in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies. The human *WDR27* gene is located on chromosome 6 (6q16.1) and encodes a protein with seven WD40 repeats. Limited studies suggest its potential involvement in ciliary function, developmental pathways, and DNA repair, though mechanistic details are scarce.
Emerging research links *WDR27* genetic variants to developmental disorders and cancer, hinting at its role in cellular homeostasis. Its expression profile in tissues like the brain, kidneys, and testes implies organ-specific functions. Current efforts focus on elucidating its interactome and post-translational modifications to define its physiological and pathological relevance. Recombinant WDR27 tools are crucial for structural studies, antibody development, and screening for therapeutic targets, offering avenues to explore its utility in diagnostics or disease modulation. However, comprehensive characterization remains a priority to bridge knowledge gaps in its molecular mechanisms.
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