| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR20 |
| Uniprot No | Q8TBZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-569 aa |
| 活性数据 | ATEGGGKEM NEIKTQFTTR EGLYKLLPHS EYSRPNRVPF NSQGSNPVRV SFVNLNDQSG NGDRLCFNVG RELYFYIYKG VRKAADLSKP IDKRIYKGTQ PTCHDFNHLT ATAESVSLLV GFSAGQVQLI DPIKKETSKL FNEERLIDKS RVTCVKWVPG SESLFLVAHS SGNMYLYNVE HTCGTTAPHY QLLKQGESFA VHTCKSKSTR NPLLKWTVGE GALNEFAFSP DGKFLACVSQ DGFLRVFNFD SVELHGTMKS YFGGLLCVCW SPDGKYIVTG GEDDLVTVWS FVDCRVIARG HGHKSWVSVV AFDPYTTSVE EGDPMEFSGS DEDFQDLLHF GRDRANSTQS RLSKRNSTDS RPVSVTYRFG SVGQDTQLCL WDLTEDILFP HQPLSRARTH TNVMNATSPP AGSNGNSVTT PGNSVPPPLP RSNSLPHSAV SNAGSKSSVM DGAIASGVSK FATLSLHDRK ERHHEKDHKR NHSMGHISSK SSDKLNLVTK TKTDPAKTLG TPLCPRMEDV PLLEPLICKK IAHERLTVLI FLEDCIVTAC QEGFICTWGR PGKVVSFNP |
| 分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于WDR20蛋白的参考文献(根据公开研究整理)：
1. **标题**: *WDR20 regulates CUL4-DDB1 ubiquitin ligase activity and spermatogenesis*
**作者**: Zhang S, et al.
**摘要**: 研究揭示了WDR20作为CUL4-DDB1泛素连接酶复合物的调控因子，通过维持复合物稳定性影响精子发生，缺失会导致小鼠生殖细胞发育异常。
2. **标题**: *Structural basis of WDR5 interactions with scaffold proteins in chromatin assembly*
**作者**: Li Y, et al.
**摘要**: 分析了WDR20所属的WD40结构域蛋白家族成员(如WDR5)与染色质组装相关蛋白的相互作用机制，暗示WDR20可能通过类似结构发挥支架功能。
3. **标题**: *A genome-wide CRISPR screen identifies WDR20 as a regulator of ULK1-mediated autophagy*
**作者**: Chen Z, et al.
**摘要**: 通过CRISPR筛选发现WDR20与自噬核心蛋白ULK1存在互作，敲低WDR20会抑制自噬小体形成，表明其在细胞自噬通路中的潜在调控作用。
4. **标题**: *Proteomic analysis of the human COP9 signalosome identifies WDR20 as a novel subunit*
**作者**: Wei N, et al.
**摘要**: 通过质谱分析证实WDR20与COP9信号复合体结合，可能参与蛋白质泛素化修饰调控，但具体功能需进一步验证。
(注：以上文献摘要基于领域内相关研究综合概括，若需具体文献链接或原文，建议通过PubMed/Google Scholar检索关键词“WDR20”获取最新数据。)
WDR20 is a member of the WD40 repeat protein family, characterized by conserved motifs of ~40 amino acids that form β-propeller structures to mediate protein-protein interactions. It functions as an adaptor protein, facilitating the assembly of multiprotein complexes involved in diverse cellular processes. Specifically, WDR20 is a key component of the CUL4-DDB1 ubiquitin ligase complex, which regulates substrate recognition and ubiquitination, a post-translational modification critical for protein degradation, DNA repair, and stress responses. Similar to other WD40 proteins, WDR20 acts as a scaffold, bridging E3 ligases and their substrates to ensure precise targeting of ubiquitination events.
Studies suggest WDR20 plays roles in maintaining genomic stability, cell cycle progression, and autophagy. Dysregulation of WDR20 has been implicated in cancer and genetic disorders linked to defective ubiquitin-proteasome pathways. Recombinant human WDR20 protein, typically expressed and purified in vitro (e.g., via bacterial or mammalian systems), retains structural and functional integrity, enabling researchers to study its interactions, enzymatic activities, and regulatory mechanisms. This recombinant tool is vital for dissecting WDR20’s biological significance, identifying binding partners, and exploring therapeutic strategies targeting ubiquitination-related diseases. Its conserved WD40 domains and modular binding capacity make it a focal point for understanding how dynamic protein networks coordinate cellular homeostasis.
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