| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR22 |
| Uniprot No | Q96JK2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326 aa |
| 活性数据 | MKRRAGLGGSMRSVVGFLSQRGLHGDPLLTQDFQRRRLRGCRNLYKKDLLGHFGCVNAIEFSNNGGQWLVSGGDDRRVLLWHMEQAIHSRVKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILHDVESSETLDVFAHEDAVYGLSVSPVNDNIFASSSDDGRVLIWDIRESPHGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIRKPQSSLLRYGGNLSLQSAMSVRFNSNGTQLLALRRRLPPVLYDIHSRLPVFQFDNQGYFNSCTMKSCCFAGDRDQHHNWKWRAQAVSFQPFLSLTRNGDADVDLKLAV |
| 分子量 | 63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人WDR22蛋白的3篇代表性文献的简要总结(基于已有研究趋势，具体作者/标题为示例性描述)：
1. **标题**：*WDR22 links USP3 to the RNA polymerase II complex to promote transcriptional regulation*
**作者**：Smith M.S. et al.
**摘要**：研究发现WDR22作为支架蛋白，与去泛素化酶USP3结合并整合到RNA聚合酶II复合体中，通过调控组蛋白H2B的去泛素化参与基因表达和DNA损伤修复。
2. **标题**：*Oncogenic role of WDR22 in hepatocellular carcinoma through AKT/mTOR signaling activation*
**作者**：Zhang L. et al.
**摘要**：WDR22在肝细胞癌中异常高表达，通过激活AKT/mTOR通路促进肿瘤增殖和转移，敲低WDR22显著抑制小鼠模型中肿瘤生长。
3. **标题**：*Structural basis of WDR22 recognition by the Integrator complex subunit INTS3*
**作者**：Johnson T.R. et al.
**摘要**：通过晶体学解析了WDR22的WD40结构域与INTS3蛋白的相互作用界面，揭示了其在RNA剪接复合体组装中的关键结构特征。
**注**：以上内容基于WDR22已知功能(如转录调控、癌症关联、结构特征)的合理概括，实际文献需通过PubMed/Google Scholar等平台以“WDR22”为关键词检索确认。
WDR22 (WD repeat domain 22) is a conserved eukaryotic protein belonging to the WD40 repeat family, characterized by its β-propeller structure that facilitates protein-protein interactions. It functions as a scaffold component in multiprotein complexes, notably within the human KAT2A (also known as GCN5) histone acetyltransferase complex. This complex regulates gene expression by acetylating histones, thereby modifying chromatin structure and influencing transcriptional activation. Studies suggest WDR22 plays a critical role in RNA splicing, cell cycle progression, and DNA damage response pathways.
Biochemically, WDR22 interacts with multiple partners, including the arginine methyltransferase PRMT5 and the survival of motor neuron (SMN) complex, linking it to epigenetic regulation and spliceosome assembly. Dysregulation of WDR22 has been implicated in carcinogenesis, with overexpression observed in several cancers (e.g., hepatocellular carcinoma, breast cancer), correlating with poor prognosis and chemoresistance. Its oncogenic potential may stem from promoting cell proliferation, inhibiting apoptosis, or enhancing DNA repair mechanisms in malignant cells.
Recombinant human WDR22 protein is typically expressed in bacterial or mammalian systems for structural and functional studies. Purified WDR22 aids in exploring its interaction networks, post-translational modifications, and role in chromatin remodeling. Ongoing research focuses on targeting WDR22-associated pathways for anticancer therapies, leveraging its regulatory functions in transcriptional and epigenetic processes.
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