| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR21C |
| Uniprot No | Q8NA75 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395 aa |
| 活性数据 | MESKRPRLLEEADKQKKTVRVGLNAPSMLRKNQLGFLRFANYCRIARELRVSCMQRKKVQIHSWDPSSLASDRFNRILANTNTDQLFTVNQVEAGGSKYGIITMRGLTTPELRVYPHKTLYVPNRKVNSMCWASLNHLDSHLLLCFVGLADTPSCAVLLPASLFIGSFPGMRRPGMLCSFQIPDAWSCAWSLSIHAYHSFSTGLSQQVLLTNVVTGHQQSFGTSSDVLAQQFAIMTPLLFNGCRSGEIFGIDLRCGNQGSGWKAICLSHDSAVTSLQILQDGQFLVSSDMTGTIKLWDLRATKCVTQYEGHVNNSAYLPVHVNEEEGVVAAVGQDCYTRIWSLRHGHLLTTIPSPYPASENDIPSVAFSSRLGGFRGAPGLLMAVREDLYCFSYG |
| 分子量 | 70.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前关于WDR21C蛋白的研究较为有限，以下是假设的参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
1. **文献名称**：*"Structural and functional characterization of WDR21C as a substrate receptor for CRL4 ubiquitin ligase"*
**作者**：Smith, J. et al. (2021)
**摘要**：本研究解析了WDR21C的晶体结构，发现其作为CRL4泛素连接酶的底物受体，通过WD40结构域招募靶蛋白，参与DNA损伤修复通路。
2. **文献名称**：*"WDR21C deficiency disrupts neuronal development via impaired autophagy-lysosomal pathway"*
**作者**：Li, X. & Zhang, Y. (2020)
**摘要**：利用CRISPR敲除技术，揭示了WDR21C通过调控自噬相关蛋白(如LC3)的泛素化，影响神经元发育异常，提示其与神经退行性疾病的潜在关联。
3. **文献名称**：*"Proteomic screening identifies WDR21C as a novel interactor of the mTOR signaling complex"*
**作者**：Chen, R. et al. (2019)
**摘要**：通过蛋白质组学分析，发现WDR21C与mTOR复合物相互作用，可能通过调节其稳定性影响细胞代谢和肿瘤生长。
4. **文献名称**：*"WDR21C: A potential biomarker for colorectal cancer progression linked to TP53 mutations"*
**作者**：Wang, L. et al. (2022)
**摘要**：临床样本分析显示，WDR21C在结直肠癌中高表达，并与TP53突变状态正相关，可能作为预后标志物和治疗靶点。
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**建议**：
可通过 **PubMed** 或 **Google Scholar** 搜索关键词“WDR21C”或别名“DCAF15”，结合“CRL4”、“ubiquitination”等术语获取最新研究。若文献有限，可扩展至WD40蛋白家族的功能综述。
**Background of Recombinant Human WDR21C Protein**
WDR21C (WD repeat domain-containing protein 21C) is a member of the WD40 repeat protein family, characterized by conserved WD40 motifs that facilitate protein-protein interactions and multiprotein complex assembly. These proteins often act as scaffolds, regulating diverse cellular processes such as signal transduction, chromatin remodeling, and intracellular trafficking. The WDR21C gene is located on human chromosome 9q34.3. encoding a protein with multiple WD40 repeats predicted to form a β-propeller structure.
Although the precise biological role of WDR21C remains under investigation, studies suggest involvement in cellular pathways linked to autophagy, vesicular transport, or transcriptional regulation. Its WD40 domains likely mediate interactions with partner proteins, potentially influencing ubiquitination processes or cytoskeletal dynamics. However, functional data are limited, and its exact mechanism remains unclear.
Recombinant human WDR21C protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling biochemical and structural studies. It is utilized to investigate binding partners, enzymatic activity, or regulatory roles in disease contexts, such as cancer or neurodegenerative disorders. Challenges in recombinant production include maintaining solubility and structural integrity due to the repetitive nature of WD40 domains. Further research is needed to unravel its physiological significance and therapeutic potential.
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