| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR23 |
| Uniprot No | Q8TEB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-546 aa |
| 活性数据 | MGSRNSSSAG SGSGDPSEGL PRRGAGLRRS EEEEEEDEDV DLAQVLAYLL RRGQVRLVQG GGAANLQFIQ ALLDSEEEND RAWDGRLGDR YNPPVDATPD TRELEFNEIK TQVELATGQL GLRRAAQKHS FPRMLHQRER GLCHRGSFSL GEQSRVISHF LPNDLGFTDS YSQKAFCGIY SKDGQIFMSA CQDQTIRLYD CRYGRFRKFK SIKARDVGWS VLDVAFTPDG NHFLYSSWSD YIHICNIYGE GDTHTALDLR PDERRFAVFS IAVSSDGREV LGGANDGCLY VFDREQNRRT LQIESHEDDV NAVAFADISS QILFSGGDDA ICKVWDRRTM REDDPKPVGA LAGHQDGITF IDSKGDARYL ISNSKDQTIK LWDIRRFSSR EGMEASRQAA TQQNWDYRWQ QVPKKAWRKL KLPGDSSLMT YRGHGVLHTL IRCRFSPIHS TGQQFIYSGC STGKVVVYDL LSGHIVKKLT NHKACVRDVS WHPFEEKIVS SSWDGNLRLW QYRQAEYFQD DMPESEECAS APAPVPQSST PFSSPQ |
| 分子量 | 61.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR23蛋白的3篇参考文献的简要信息(注：以下内容为模拟生成，部分文献可能并非真实存在，仅供参考)：
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1. **文献名称**: *WDR23 mediates proteostasis recovery via the NRF2-KEAP1 pathway under oxidative stress*
**作者**: Tanaka, K., et al. (2018)
**摘要**: 研究揭示了WDR23作为CUL3泛素连接酶的底物受体，通过调控NRF2的稳定性参与氧化应激反应。实验表明，WDR23缺失导致NRF2积累，增强抗氧化基因表达，提示其在细胞应激适应中的关键作用。
2. **文献名称**: *Structural basis of WDR23-DDB1 interaction in the context of CRL4 ubiquitin ligase complexes*
**作者**: Liu, X., & Wei, S. (2020)
**摘要**: 通过冷冻电镜解析了WDR23与CUL4-DDB1复合物的结构，阐明其WD40结构域如何识别并结合DDB1.进一步揭示WDR23在CRL4泛素连接酶复合体中招募特定底物进行降解的分子机制。
3. **文献名称**: *WDR23 modulates genomic stability through regulating p53 degradation in cancer cells*
**作者**: Chen, L., et al. (2021)
**摘要**: 研究发现WDR23通过与CUL4-DDB1复合体结合，介导肿瘤抑制蛋白p53的泛素化降解。敲低WDR23可增强p53稳定性，抑制癌细胞增殖，表明其作为潜在癌症治疗靶点的价值。
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**注意**：以上文献信息为生成示例，具体内容需以实际发表的学术论文为准。建议通过PubMed或Web of Science等数据库检索最新研究。
**Background of Recombinant Human WDR23 Protein**
The recombinant human WDR23 (WD repeat domain protein 23) is a conserved scaffold protein belonging to the WD40-repeat protein family, characterized by its β-propeller structure formed by repeating WD40 motifs. These motifs enable versatile protein-protein interactions, allowing WDR23 to act as a substrate-recognition component of CUL4-DDB1 E3 ubiquitin ligase complexes. This role positions WDR23 in the ubiquitin-proteasome system, where it facilitates targeted degradation of specific proteins to regulate cellular processes.
Functionally, WDR23 is implicated in oxidative stress response pathways. It interacts with the NRF2 transcription factor, a master regulator of antioxidant genes. By modulating NRF2 stability through ubiquitination, WDR23 influences cellular defense against reactive oxygen species (ROS). Additionally, WDR23 is linked to DNA repair, cell cycle control, and genomic stability, with studies suggesting its involvement in repairing UV-induced DNA damage via the DDB1-CUL4 complex.
Research highlights WDR23’s dual regulatory roles in health and disease. Dysregulation of WDR23 expression or activity is associated with cancers, neurodegenerative disorders (e.g., Alzheimer’s disease), and aging-related conditions. Recombinant human WDR23 protein is widely used in biochemical studies to dissect its molecular mechanisms, interactions, and pathological relevance, offering potential therapeutic insights. Its conserved structure-function relationships across species further underscore its biological significance in cellular homeostasis.
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