| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR21A |
| Uniprot No | Q8WV16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495 aa |
| 活性数据 | MNKSRWQSRRRHGRRSHQQNPWFRLRDSEDRSDSRAAQPAHDSGHGDDESPSTSSGTAGTSSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRKKIARMGFNASSMLRKSQLGFLNVTNYCHLAHELRLSCMERKKVQIRSMDPSALASDRFNLILADTNSDRLFTVNDVKVGGSKYGIINLQSLKTPTLKVFMHENLYFTNRKVNSVCWASLNHLDSHILLCLMGLAETPGCATLLPASLFVNSHPGIDRPGMLCSFRIPGAWSCAWSLNIQANNCFSTGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQGKGWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNEYAYLPLHVHEEEGILVAVGQDCYTRIWSLHDARLLRTIPSPYPASKADIPSVAFSSRLGGSRGAPGLLMAVGQDLYCYSYS |
| 分子量 | 71.7kDa kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR21A蛋白的3篇假设性参考文献及简要内容概括：
1. **"WDR21A regulates CUL4-DDB1 ubiquitin ligase complex assembly in DNA repair"**
*Authors: Smith J., Wang L. et al. (2020)*
**摘要**：本研究报道WDR21A作为CUL4-DDB1泛素连接酶复合体的调控因子，通过重组蛋白实验揭示其参与DNA损伤修复的分子机制，证明其通过促进底物泛素化维持基因组稳定性。
2. **"Recombinant WDR21A expression and its role in neuronal differentiation"**
*Authors: Kim S., Patel R. et al. (2019)*
**摘要**：利用重组WDR21A蛋白体外模型，发现其通过调控Notch信号通路影响神经干细胞分化，敲低WDR21A导致神经元成熟延迟，提示其在神经发育中的潜在作用。
3. **"Structural insights into WDR21A-mediated protein-protein interactions"**
*Authors: Gonzalez M., Zhang Y. et al. (2021)*
**摘要**：通过晶体学解析重组WDR21A的WD40结构域三维结构，揭示其介导多蛋白复合物组装的关键界面，为靶向WDR21A的疾病治疗策略提供结构基础。
*注：以上为模拟文献，实际研究中建议通过PubMed/Google Scholar以“WDR21A”及“recombinant WDR21A protein”为关键词检索最新实证论文。*
WDR21A (WD repeat-containing protein 21A) is a member of the WD-repeat protein family, characterized by conserved tandem repeats of ~40–60 amino acids typically ending with a tryptophan-aspartate (WD) motif. These proteins are involved in diverse cellular processes, such as signal transduction, protein-protein interactions, and chromatin remodeling. WDR21A is thought to act as a scaffold or adaptor in multiprotein complexes, facilitating molecular interactions. It has been linked to the CUL4-DDB1 E3 ubiquitin ligase complex, which mediates substrate recognition in ubiquitination pathways, suggesting a role in protein degradation and post-translational regulation.
Functionally, WDR21A may influence cellular stress responses and genomic stability. Studies indicate its involvement in DNA repair mechanisms, potentially through interactions with damaged DNA-binding proteins. Dysregulation of WD-repeat proteins, including WDR21A, has been associated with cancer and neurodegenerative disorders, though its specific pathogenic mechanisms remain under investigation. Recombinant human WDR21A protein is commonly expressed in bacterial or mammalian systems for structural and functional studies. Its recombinant form enables biochemical assays, interaction mapping, and antibody production, aiding research into its physiological roles and therapeutic potential. Further exploration of WDR21A could clarify its contributions to ubiquitin-mediated proteostasis and disease pathways.
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