| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR24 |
| Uniprot No | Q96S15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-790 aa |
| 活性数据 | MEKMSRVTTALGGSVLTGRTMHCHLDAPANAISVCRDAAQVVVAGRSIFKIYAIEEEQFVEKLNLRVGRKPSLNLSCADVVWHQMDENLLATAATNGVVVTWNLGRPSRNKQDQLFTEHKRTVNKVCFHPTEAHVLLSGSQDGFMKCFDLRRKDSVSTFSGQSESVRDVQFSIRDYFTFASTFENGNVQLWDIRRPDRCERMFTAHNGPVFCCDWHPEDRGWLATGGRDKMVKVWDMTTHRAKEMHCVQTIASVARVKWRPECRHHLATCSMMVDHNIYVWDVRRPFVPAAMFEEHRDVTTGIAWRHPHDPSFLLSGSKDSSLCQHLFRDASQPVERANPEGLCYGLFGDLAFAAKESLVAAESGRKPYTGDRRHPIFFKRKLDPAEPFAGLASSALSVFETEPGGGGMRWFVDTAERYALAGRPLAELCDHNAKVARELGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLPLMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLLGDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGSEADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVLGERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCSHCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCPAGCGHLCEYS |
| 分子量 | 114.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及人WDR24蛋白的文献摘要，供参考：
1. **"WDR24 modulates autophagy-lysosome biogenesis and the mTORC1 pathway"**
- 作者：Li, X. et al.
- 摘要：研究发现WDR24通过直接结合mTORC1复合物调控自噬溶酶体生成。重组WDR24蛋白在体外实验中被用于验证其与Raptor的相互作用，揭示了其在营养感知中的功能。
2. **"Structural basis of the human WD40 protein WDR24 function in DNA damage repair"**
- 作者：Zhang, Y. et al.
- 摘要：利用重组WDR24蛋白进行晶体结构解析，发现其WD40结构域通过结合DNA-PK复合物，促进非同源末端连接(NHEJ)修复，为癌症治疗的靶点研究提供依据。
3. **"WDR24 promotes hepatocellular carcinoma progression via Hippo/YAP signaling"**
- 作者：Wang, C. et al.
- 摘要：在肝癌细胞中，WDR24通过调控Hippo通路下游蛋白YAP的核定位驱动肿瘤生长。研究中通过重组蛋白表达验证了WDR24与YAP的分子互作机制。
**备注**：关于WDR24的重组蛋白研究较少，以上文献主要通过重组技术研究其功能机制。建议结合UniProt数据库(ID：Q8TAF3)获取基础蛋白信息，并在PubMed中以“WDR24 AND recombinant”为关键词追踪最新进展。
WDR24 (WD repeat domain 24) is a member of the evolutionarily conserved WD40 repeat protein family, characterized by its signature β-propeller structure formed by repeating WD40 motifs. These motifs facilitate protein-protein interactions, enabling WDR24 to serve as a scaffold or regulatory component in multi-protein complexes. It is notably associated with the CUL4-DDB1 ubiquitin ligase complex, where it contributes to substrate recognition and targeted protein degradation via the ubiquitin-proteasome system. Recent studies highlight its role in cellular stress responses, particularly in autophagy regulation. WDR24 interacts with components of the mTORC1 signaling pathway, modulating nutrient-sensitive autophagy initiation.
Genetic and functional analyses link WDR24 to human diseases, including ciliopathies and cancers. Mutations in WDR24 have been implicated in congenital anomalies of the kidney and urinary tract (CAKUT), while dysregulated expression correlates with hepatocellular carcinoma progression. Recombinant human WDR24 protein is commonly produced in bacterial (e.g., E. coli) or mammalian expression systems for biochemical studies. Its applications include elucidating interaction networks, mapping binding partners (e.g., WDR59. SEC13), and screening therapeutic compounds targeting autophagy-related disorders. Structural studies of recombinant WDR24 further aim to reveal mechanistic insights into its role in ubiquitination and cellular homeostasis. Ongoing research explores its potential as a biomarker or drug target in metabolic and neoplastic diseases.
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