| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR16 |
| Uniprot No | Q8N1V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-620 aa |
| 活性数据 | MDNKISPEAQ VAELELDAVI GFNGHVPTGL KCHPDQEHMI YPLGCTVLIQ AINTKEQNFL QGHGNNVSCL AISRSGEYIA SGQVTFMGFK ADIILWDYKN RELLARLSLH KGKIEALAFS PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATNVIFSRCR DEMFMTAGNG TIRVWELDLP NRKIWPTECQ TGQLKRIVMS IGVDDDDSFF YLGTTTGDIL KMNPRTKLLT DVGPAKDKFS LGVSAIRCLK MGGLLVGSGA GLLVFCKSPG YKPIKKIQLQ GGITSITLRG EGHQFLVGTE ESHIYRVSFT DFKETLIATC HFDAVEDIVF PFGTAELFAT CAKKDIRVWH TSSNRELLRI TVPNMTCHGI DFMRDGKSII SAWNDGKIRA FAPETGRLMY VINNAHRIGV TAIATTSDCK RVISGGGEGE VRVWQIGCQT QKLEEALKEH KSSVSCIRVK RNNEECVTAS TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE VFDGTVIREL EGSLSGSING MDITQEGVHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ YIVSVSADGA ILRWKYPYTS |
| 分子量 | 68.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR16蛋白的3-4条参考文献示例(文献信息为概括性描述，非真实存在的原文)：
1. **文献名称**：*Structural and Functional Analysis of WDR16 Reveals Its Role in Cilia Assembly*
**作者**：Smith A, Johnson B.
**摘要**：本研究解析了WDR16蛋白的晶体结构，揭示了其WD40重复结构域与CEP89蛋白的相互作用机制，证明其在纤毛发生中的关键作用，并验证了重组WDR16在体外促进微管组装的活性。
2. **文献名称**：*WDR16 Regulates Transcriptional Coactivation via Interactions with TP73 and p300*
**作者**：Chen L, Wang Y.
**摘要**：通过免疫共沉淀和荧光素酶实验，发现重组人WDR16蛋白与转录因子TP73及组蛋白乙酰转移酶p300形成复合物，调控靶基因表达，暗示其在DNA损伤修复通路中的潜在功能。
3. **文献名称**：*Loss of WDR16 in Zebrafish Model Disrupts Ciliogenesis and Causes Renal Cysts*
**作者**：Adams R, et al.
**摘要**：利用CRISPR技术构建WDR16敲除斑马鱼模型，观察到纤毛缺陷和肾脏囊肿表型;重组人WDR16蛋白回补实验部分恢复表型，表明其进化保守的纤毛相关功能。
4. **文献名称**：*High-Yield Production of Recombinant Human WDR16 in E. coli and Its Biochemical Characterization*
**作者**：Garcia M, et al.
**摘要**：报道了一种高效的原核表达系统制备重组WDR16蛋白的方法，优化纯化条件后获得高纯度蛋白，并验证其与已知结合蛋白的相互作用，为后续功能研究提供工具。
(注：以上文献为模拟内容，实际研究中建议通过PubMed或Google Scholar以“WDR16”、“重组蛋白”等关键词检索最新论文。)
WDR16 (WD repeat domain 16) is a member of the evolutionarily conserved WD40 repeat protein family, characterized by tandem repeats of ~40 amino acids typically forming β-propeller structures to mediate protein-protein interactions. In humans, WDR16 is broadly expressed and localized to cytoplasm, cilia, and centrosomes, suggesting roles in cellular processes like cell cycle regulation, ciliogenesis, and signal transduction. Studies link WDR16 to primary cilia function, where it interacts with transition zone proteins (e.g., CEP290) to regulate ciliary assembly and trafficking, implicating its potential involvement in ciliopathies. Its interaction with GTPases and kinases further supports regulatory functions in cellular dynamics. Recombinant WDR16 protein, generated via heterologous expression systems (e.g., E. coli, mammalian cells), enables biochemical and structural analyses to dissect its interaction networks and mechanistic contributions to health and disease. Current research explores its associations with developmental disorders, cancer progression, and ciliary dysfunction, though its precise molecular roles remain under investigation. The recombinant form serves as a critical tool for deciphering WDR16's structure-function relationships and therapeutic relevance.
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