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Recombinant Human VMD2 Protein

  • 中文名: 重组人(VMD2)蛋白
  • 别    名: BEST1; VMD2; Bestrophin-1; TU15B; Vitelliform macular dystrophy Protein 2
货号: PAX2000-12465
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点VMD2
Uniprot NoO76090
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-604 aa
活性数据MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRVSFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR
分子量95.5 kDa
蛋白标签GST-tag at N-terminal
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人VMD2(Bestrophin-1)蛋白的假设性参考文献示例(基于研究领域常见方向,实际文献需根据具体数据库检索确认):

1. **文献名称**: "Expression and Functional Characterization of Recombinant Human Bestrophin-1 in HEK293 Cells"

**作者**: Smith A, et al.

**摘要**: 研究通过哺乳动物细胞系统(HEK293)成功表达重组人Bestrophin-1蛋白,并证实其在细胞膜上的氯离子通道功能,揭示了其在视网膜色素上皮细胞中的生理作用。

2. **文献名称**: "Structural Insights into the VMD2 Protein: Cryo-EM Analysis of Recombinant Bestrophin-1"

**作者**: Zhang L, et al.

**摘要**: 首次报道重组人Bestrophin-1蛋白的冷冻电镜结构,解析其四聚体构象及钙离子结合位点,为理解VMD2突变导致遗传性黄斑变性的机制提供结构基础。

3. **文献名称**: "Functional Rescue of Bestrophin-1 Mutants Using Recombinant Protein Delivery in Patient-Derived RPE Cells"

**作者**: Johnson R, et al.

**摘要**: 构建重组Bestrophin-1蛋白并通过脂质体递送至患者来源的视网膜色素上皮(RPE)细胞,部分恢复了突变细胞的离子转运活性,验证了蛋白替代疗法的潜在可行性。

4. **文献名称**: "In Vitro Reconstitution of Bestrophin-1 Interaction with the Calcium Signaling Pathway"

**作者**: Müller S, et al.

**摘要**: 利用重组Bestrophin-1蛋白建立体外实验模型,发现其与钙调蛋白的互作关系,揭示了其在细胞钙稳态调控中的双重功能(离子通道与信号调节因子)。

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**注**: 以上内容为示例性概括,实际文献需通过PubMed/Google Scholar检索关键词(如:"recombinant human BEST1 protein"、"VMD2 expression")获取。关注研究方向可包括:蛋白功能验证、结构解析、疾病模型治疗或信号通路机制。


背景信息

**Background of Recombinant Human VMD2 (Bestrophin-1) Protein**

The human *VMD2* gene, located on chromosome 11q12.3. encodes bestrophin-1. a transmembrane protein predominantly expressed in the retinal pigment epithelium (RPE). Functioning as a chloride channel or regulator, bestrophin-1 plays a critical role in maintaining ionic homeostasis, fluid transport, and phagocytic activity within the retina, essential for visual function. Mutations in *VMD2* are causally linked to Best vitelliform macular dystrophy (BVMD), a hereditary retinal disorder characterized by abnormal lipofuscin accumulation, RPE degeneration, and progressive vision loss.

Recombinant human VMD2 protein is engineered to study its structural, functional, and pathological mechanisms. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), this protein enables in vitro analysis of ion channel activity, interaction partners (e.g., Ca²⁺ signaling proteins), and disease-associated mutant variants. Its recombinant form is vital for elucidating how *VMD2* mutations disrupt cellular processes, contributing to macular degeneration. Additionally, it serves as a tool for drug screening, gene therapy development, and functional rescue studies aimed at restoring chloride transport defects. Research on recombinant bestrophin-1 continues to advance therapeutic strategies, including small molecule correctors or adeno-associated virus (AAV)-based gene delivery, to address Best disease and related retinopathies.


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