| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | U2AF2 |
| Uniprot No | P26368 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-475 aa |
| 活性数据 | SDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW |
| 分子量 | 53.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人U2AF2蛋白的参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **文献名称**: "Structural basis of RNA recognition by the human U2AF2 protein in 3′ splice site selection"
**作者**: Xue, Y., et al.
**摘要**: 解析了重组人U2AF2蛋白的晶体结构，阐明其通过双锌指结构域识别pre-mRNA上特定多聚嘧啶序列的分子机制，揭示了其在剪接位点选择中的关键作用。
2. **文献名称**: "Functional interplay between U2AF2 and SF3b complex in spliceosome assembly"
**作者**: Wang, Z., et al.
**摘要**: 利用重组人U2AF2蛋白进行体外结合实验，证明其与剪接体SF3b复合物的动态互作，并调控早期剪接体的组装过程。
3. **文献名称**: "Aberrant U2AF2 phosphorylation promotes cancer-associated splicing variants"
**作者**: Lee, C., & Kaneko, S.
**摘要**: 研究重组U2AF2蛋白的磷酸化修饰对其剪接功能的影响，发现特定激酶介导的磷酸化异常导致肿瘤相关mRNA剪接变体的产生。
4. **文献名称**: "High-yield expression and purification of recombinant human U2AF2 for biochemical assays"
**作者**: Müller, T., et al.
**摘要**: 开发了一种基于大肠杆菌的重组U2AF2蛋白高效表达纯化方案，并验证其在RNA结合活性实验中的应用。
(注：以上文献为示例性质，具体文献需通过PubMed或Web of Science等平台以关键词“U2AF2”、“recombinant”检索核实。)
U2AF2 (U2 Small Nuclear RNA Auxiliary Factor 2), also known as U2AF65. is a critical protein involved in pre-mRNA splicing, a fundamental process for gene expression in eukaryotic cells. As a component of the U2AF complex, it plays a pivotal role in recognizing the polypyrimidine tract near the 3' splice site during the early stages of spliceosome assembly. Structurally, U2AF2 contains two RNA recognition motifs (RRMs) and a C-terminal RS domain, enabling interactions with both RNA and other splicing factors.
This protein is essential for constitutive splicing and participates in alternative splicing events, influencing mRNA diversity and cellular function. Dysregulation of U2AF2 has been linked to various diseases, including myelodysplastic syndromes and cancers, where mutations or aberrant expression can disrupt splicing fidelity. Recombinant U2AF2 proteins are widely used to study spliceosome mechanics, investigate splicing-related pathologies, and screen therapeutic compounds targeting splicing anomalies.
Produced via heterologous expression systems (e.g., E. coli or mammalian cells), recombinant U2AF2 retains structural integrity and biochemical activity, making it a valuable tool for in vitro assays, structural studies, and disease modeling. Its functional conservation across species underscores its evolutionary significance in RNA processing.
×
