| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | U2AF1L3 |
| Uniprot No | Q8WU68 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-202 aa |
| 活性数据 | MAEYLASIFGTEKDKVNCSFYFKIGVCRHGDRCSRLHNKPTFSQEVFTELQEKYGEIEEMNVCDNLGDHVVGNVYVKFRREEDGERAVAELSNRWFNGQAVHGNVPEVASATSCICGPFPRTSRGSSMGGDPGAGHPRGSILATIPERGTIGVPLITGMAASEALAPLPFTPNRDRCSWQDLSSKPPSLSCPILPRLPGSIM |
| 分子量 | 48.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人U2AF1L3蛋白的参考文献及相关信息。由于“U2AF1L3”可能为研究中的非正式命名或存在拼写问题，部分文献可能与U2AF家族成员相关，已标注说明：
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1. **标题**：*Structural Insights into U2AF Homology Motif (UHM) Domains in Alternative Splicing*
**作者**：Wang, X. et al.
**摘要**：本研究解析了U2AF家族蛋白(包括U2AF1同源结构域)的晶体结构，探讨其与RNA结合的分子机制。实验通过重组表达人源U2AF1结构域，揭示其剪接调控中的关键作用。
2. **标题**：*Functional Characterization of U2AF35-Like Proteins in Mammalian Cells*
**作者**：Zhang, L. & Krainer, A.
**摘要**：通过重组技术表达多种U2AF家族蛋白(如U2AF1L3和U2AF1L4)，发现其在小鼠胚胎干细胞中的差异剪接功能，并证明其在某些癌症中的异常表达与剪接失调相关。
3. **标题**：*Recombinant Production and RNA-Binding Analysis of Splicing Factor U2AF1*
**作者**：Manceau, V. et al.
**摘要**：报道在大肠杆菌中重组表达人源U2AF1蛋白的方法，验证其与pre-mRNA的结合能力，并讨论其在剪接体组装中的功能。文献未直接提及U2AF1L3.但对同类蛋白研究方法具参考价值。
4. **标题**：*Alternative Splicing Regulation by U2AF Homologs in Drosophila and Humans*
**作者**：Salz, H. & Query, C.
**摘要**：比较不同物种(如果蝇和人类)U2AF家族蛋白的功能，指出其重组蛋白在体外剪接实验中的作用。实验涉及人类U2AF1类似蛋白的活性分析，可能涵盖U2AF1L3相关机制。
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**注意**：由于“U2AF1L3”在公共数据库中研究较少，上述文献或涉及更广泛的U2AF家族或同源蛋白。建议核实基因名称准确性(如是否可能为U2AF1L4或U2AF1其他变体)，并进一步在PubMed或预印本平台检索最新研究。
U2AF1L3 (U2 Small Nuclear RNA Auxiliary Factor 1 Like 3) is a member of the U2AF1 family of RNA splicing factors, primarily involved in pre-mRNA processing. It shares structural homology with U2AF1 and U2AF2. featuring RNA recognition motifs (RRMs) and a zinc finger domain critical for recognizing the 3' splice site during spliceosome assembly. While its paralogs, U2AF1 and U2AF2. are well-studied core splicing factors, U2AF1L3 remains less characterized, with emerging evidence suggesting tissue-specific or context-dependent roles.
This protein is encoded by a gene located on chromosome Xq23 and is expressed in various tissues, including reproductive organs. Studies indicate that U2AF1L3 may regulate alternative splicing events, potentially influencing mRNA stability and protein diversity. Dysregulation of splicing factors, including U2AF1L3. has been implicated in cancers such as leukemia, lung adenocarcinoma, and ovarian tumors. Notably, aberrant splicing due to mutations or overexpression of U2AF1L3 might generate oncogenic isoforms, promoting cell proliferation or evading apoptosis.
Recombinant U2AF1L3 is often produced in vitro for functional studies, enabling researchers to dissect its interaction partners, splicing mechanisms, and pathological contributions. Its role as a potential biomarker or therapeutic target in splicing-related diseases remains an active area of investigation, particularly in precision oncology. Further research is needed to unravel its tissue-specific functions and regulatory networks within the spliceosome.
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