| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TZFP |
| Uniprot No | Q9Y2Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302 aa |
| 活性数据 | MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQKPKQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQRRTCNLCGS |
| 分子量 | 58.96 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人TZFP蛋白的**模拟参考文献**，基于常见相关研究方向虚构概括，仅供参考：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human TZFP in DNA-Binding Assays"*
**作者**: Zhang, L., et al.
**摘要**: 该研究成功在大肠杆菌系统中表达重组人TZFP蛋白，并通过电泳迁移率实验(EMSA)证实其特异性结合DNA启动子区域的能力，提示TZFP可能作为转录调控因子参与基因表达。
2. **文献名称**: *"TZFP Suppresses Hepatocellular Carcinoma Proliferation via Modulating Cell Cycle Pathways"*
**作者**: Li, X., et al.
**摘要**: 通过体外重组TZFP蛋白转染肝癌细胞，发现其显著抑制癌细胞增殖，机制研究表明TZFP通过下调cyclin D1表达阻滞G1/S期转化，为肝癌治疗提供潜在靶点。
3. **文献名称**: *"Structural Insights into the Zinc Finger Motifs of Recombinant TZFP by X-ray Crystallography"*
**作者**: Chen, Y., et al.
**摘要**: 首次解析重组人TZFP蛋白的晶体结构，揭示其C端锌指结构域独特的α-螺旋构象，并预测其与DNA双螺旋的互作界面，为功能研究提供结构基础。
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**注意**：以上内容为假设性示例，实际文献需通过学术数据库(如PubMed、Web of Science)搜索 **"TZFP protein"、"recombinant TZFP"** 或 **"Testis Zinc Finger Protein"** 获取。如需真实文献，请提供更详细的蛋白名称或基因编号(如NCBI Gene ID)。
**Background of Recombinant Human TZFP Protein**
The recombinant human testis zinc finger protein (TZFP), also known as testis-expressed zinc finger protein, is a transcription regulatory factor encoded by the *ZNF645* gene. Structurally, TZFP belongs to the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that mediate sequence-specific DNA or RNA interactions. TZFP is predominantly expressed in the testis, particularly during spermatogenesis, suggesting a role in germ cell development, differentiation, or transcriptional regulation.
Functionally, TZFP has been implicated in modulating gene expression by binding to promoter regions or interacting with epigenetic modifiers. Studies suggest its involvement in cellular processes such as apoptosis, cell cycle regulation, and chromatin remodeling. Dysregulation of TZFP expression has been linked to male infertility and certain cancers, highlighting its potential as a biomarker or therapeutic target.
Recombinant TZFP is typically produced in *E. coli* or mammalian expression systems, enabling in vitro studies of its molecular interactions, structural properties, and functional mechanisms. Its recombinant form facilitates research into reproductive biology, oncogenesis, and gene regulatory networks, offering insights into disease pathways and cellular homeostasis. Current efforts focus on elucidating its precise targets and regulatory roles in health and disease.
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