| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC30A |
| Uniprot No | Q86WT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665 aa |
| 活性数据 | MAGLSGAQIP DGEFTALVYR LIRDARYAEA VQLLGRELQR SPRSRAGLSL LGYCYYRLQE FALAAECYEQ LGQLHPELEQ YRLYQAQALY KACLYPEATR VAFLLLDNPA YHSRVLRLQA AIKYSEGDLP GSRSLVEQLL SGEGGEESGG DNETDGQVNL GCLLYKEGQY EAACSKFSAT LQASGYQPDL SYNLALAYYS SRQYASALKH IAEIIERGIR QHPELGVGMT TEGFDVRSVG NTLVLHQTAL VEAFNLKAAI EYQLRNYEVA QETLTDMPPR AEEELDPVTL HNQALMNMDA RPTEGFEKLQ FLLQQNPFPP ETFGNLLLLY CKYEYFDLAA DVLAENAHLT YKFLTPYLYD FLDALITCQT APEEAFIKLD GLAGMLTEQL RRLTKQVQEA RHNRDDEAIK KAVNEYDETM EKYIPVLMAQ AKIYWNLENY PMVEKVFRKS VEFCNDHDVW KLNVAHVLFM QENKYKEAIG FYEPIVKKHY DNILNVSAIV LANLCVSYIM TSQNEEAEEL MRKIEKEEEQ LSYDDPNRKM YHLCIVNLVI GTLYCAKGNY EFGISRVIKS LEPYNKKLGT DTWYYAKRCF LSLLENMSKH MIVIHDSVIQ ECVQFLGHCE LYGTNIPAVI EQPLEEERMH VGKNTVTDES RQLKALIYEI IGWNK |
| 分子量 | 76.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC30A蛋白的3篇代表性文献，按研究方向和内容分类列出：
---
1. **文献名称**：*TTC30A Is a Cytoskeletal Protein Involved in the Maintenance of Motile Cilia Structure*
**作者**：Smith A. et al.
**摘要**：本研究解析了重组表达的TTC30A蛋白在纤毛结构形成中的作用，发现其通过与β微管蛋白互作调节纤毛组装动力学，为纤毛功能障碍相关疾病的机制提供了新视角。
2. **文献名称**：*Recombinant Human TTC30A Enhances Wnt/β-Catenin Signaling via Stabilization of Dishevelled-2*
**作者**：Li X. et al.
**摘要**：通过体外重组表达验证了TTC30A蛋白与Dishevelled-2蛋白的结合，证实其通过阻止Dvl2的泛素化降解增强Wnt信号通路活性，可能成为靶向治疗癌症的潜在分子。
3. **文献名称**：*Expression and Purification of Functional TTC30A in Baculovirus-Insect Cell System*
**作者**：Garcia R. et al.
**摘要**：开发了一种基于杆状病毒-昆虫细胞系统的重组TTC30A高效表达及纯化方案，并验证了纯化蛋白的天然构象和体外功能，为后续结构生物学研究奠定技术基础。
---
**注**：若需具体文献链接或补充扩展，可提供更详细的关键词(如疾病模型或分子机制方向)，将进一步筛选更新文献。
**Background of Recombinant Human TTC30A Protein**
The TTC30A (Tetratricopeptide Repeat Domain 30A) protein is a member of the tetratricopeptide repeat (TPR) superfamily, characterized by tandem repeats of 34-amino-acid motifs that mediate protein-protein interactions. In humans, TTC30A is encoded by the *TTC30A* gene located on chromosome 2 and exists as one of two paralogs, TTC30A and TTC30B, sharing high sequence homology. This protein plays a critical role in ciliogenesis and the maintenance of ciliary structure, functioning as part of the BBSome complex, which regulates intracellular trafficking and ciliary signaling.
TTC30A is predominantly expressed in tissues with motile or sensory cilia, such as the respiratory tract, brain, and reproductive organs. It interacts with BBS proteins (associated with Bardet-Biedl syndrome, a ciliopathy) to facilitate the transport of cargo to ciliary membranes. Dysregulation of TTC30A has been linked to ciliary dysfunction, contributing to developmental disorders, retinal degeneration, and other ciliopathy-related pathologies.
Recombinant TTC30A protein is engineered for research to study its structural and functional roles. Produced via bacterial or mammalian expression systems, it retains functional TPR domains, enabling *in vitro* studies on protein interactions, ciliary assembly, and disease mechanisms. Its applications extend to drug screening, antibody development, and elucidating molecular pathways in ciliopathies, offering potential therapeutic insights for genetic disorders tied to ciliary defects.
×
