| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC29 |
| Uniprot No | Q8NA56 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475 aa |
| 活性数据 | MTTLPPLPMT RPKLTALARQ KLPCSSRKIP RSQLIKEKDD IDHYLEVNFK GLSKEEVAAY RNSYKKNICV DMLRDGYHKS FTELFALMER WDALREAARV RSLFWLQKPL EEQPDKLDYL YHYLTRAEDA ERKESFEDVH NNLYALACYF NNSEDKWVRN HFYERCFKIA QLIKIDCGKK EAEAHMHMGL LYEEDGQLLE AAEHYEAFHQ LTQGRIWKDE TGRSLNLLAC ESLLRTYRLL SDKMLENKEY KQAIKILIKA SEIAKEGSDK KMEAEASYYL GLAHLAAEEY ETALTVLDTY CKISTDLDDD LSLGRGYEAI AKVLQSQGEM TEAIKYLKKF VKIARNNFQS LDLVRASTML GDIYNEKGYY NKASECFQQA FDTTVELMSM PLMDETKVHY GIAKAHQMML TVNNYIESAD LTSLNYLLSW KESRGNIEPD PVTEEFRGST VEAVSQNSER LEELSRFPGD QKNET |
| 分子量 | 55.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的关于重组人TTC29蛋白的参考文献示例(注意：部分信息为虚构示例，建议通过学术数据库查询真实文献)：
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1. **文献名称**: "TTC29 deficiency disrupts sperm flagellar structure and causes male infertility in mice"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过基因敲除小鼠模型揭示了TTC29蛋白在精子鞭毛组装中的关键作用，其缺失导致精子运动能力丧失，提示TTC29可能作为治疗男性不育的潜在靶点。
2. **文献名称**: "Recombinant human TTC29 protein expression and purification in E. coli system"
**作者**: Liu X, et al.
**摘要**: 报道了一种利用大肠杆菌系统高效表达重组人TTC29蛋白的方法，优化纯化流程后获得高纯度蛋白，为后续结构及功能研究奠定基础。
3. **文献名称**: "Structural analysis of TTC29 reveals its role in ciliary dynein assembly"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了TTC29蛋白的三维结构，发现其通过TPR结构域与动力蛋白组分互作，调控纤毛运动机制。
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**提示**：真实文献可通过PubMed或Google Scholar以关键词 **"TTC29 protein" "recombinant"** 或 **"TTC29 spermatogenesis"** 检索，重点关注生殖生物学、蛋白结构及基因工程领域期刊。
**Background of Recombinant Human TTC29 Protein**
TTC29 (Tetratricopeptide Repeat Protein 29) is a member of the TPR-containing protein family, characterized by tandem TPR motifs that mediate protein-protein interactions. It is evolutionarily conserved and predominantly expressed in tissues with motile cilia or flagella, such as the testis and respiratory tract. Studies suggest TTC29 plays a critical role in ciliary function, particularly in spermatogenesis, where it contributes to sperm flagellum assembly and motility. Mutations in *TTC29* have been linked to male infertility due to structural defects in sperm flagella, highlighting its essential role in reproductive biology.
Recombinant human TTC29 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified TTC29 for research. Its structural properties, including the TPR domains, enable interactions with components of the ciliary axoneme and signaling pathways. Current research focuses on elucidating TTC29's molecular mechanisms in ciliogenesis, its potential association with ciliopathies (e.g., primary ciliary dyskinesia), and its utility as a biomarker for diagnosing infertility or ciliary disorders. Additionally, recombinant TTC29 aids in studying protein interactomes and screening therapeutic targets for related conditions.
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