| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC26 |
| Uniprot No | A0AVF1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-554 aa |
| 活性数据 | MMLSRAKPAV GRGVQHTDKR KKKGRKIPKL EELLSKRDFT GAITLLEFKR HVGEEEEDTN LWIGYCAFHL GDYKRALEEY ENATKEENCN SEVWVNLACT YFFLGMYKQA EAAGFKASKS RLQNRLLFHL AHKFNDEKKL MSFHQNLQDV TEDQLSLASI HYMRSHYQEA IDIYKRILLD NREYLALNVY VALCYYKLDY YDVSQEVLAV YLQQIPDSTI ALNLKACNHF RLYNGRAAEA ELKSLMDNAS SSFEFAKELI RHNLVVFRGG EGALQVLPPL VDVIPEARLN LVIYYLRQDD VQEAYNLIKD LEPTTPQEYI LKGVVNAALG QEMGSRDHMK IAQQFFQLVG GSASECDTIP GRQCMASCFF LLKQFDDVLI YLNSFKSYFY NDDIFNFNYA QAKAATGNTS EGEEAFLLIQ SEKMKNDYIY LSWLARCYIM NKKPRLAWEL YLKMETSGES FSLLQLIAND CYKMGQFYYS AKAFDVLERL DPNPEYWEGK RGACVGIFQM IIAGREPKET LREVLHLLRS TGNTQVEYMI RIMKKWAKEN RVSI |
| 分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC26蛋白的虚拟参考文献(基于当前研究领域趋势的合理推测)：
1. **文献名称**: "Mutations in TTC26 cause a novel ciliopathy associated with intrahepatic cholestasis"
**作者**: Smith A, et al.
**摘要**: 研究发现TTC26基因突变与先天性肝内胆汁淤积症相关，通过重组人TTC26蛋白的功能实验，证实其参与纤毛组装及胆汁酸运输调控。
2. **文献名称**: "Structural insights into TTC26 protein dynamics and its interaction with intraflagellar transport complex B"
**作者**: Zhang L, et al.
**摘要**: 利用重组人TTC26蛋白的晶体结构解析，揭示了该蛋白通过TPR结构域与纤毛运输复合物IFT-B结合，调控纤毛内信号转导的分子机制。
3. **文献名称**: "TTC26 dysfunction disrupts Sonic Hedgehog signaling in vertebrate ciliogenesis models"
**作者**: Johnson R, et al.
**摘要**: 在小鼠和斑马鱼模型中，重组人TTC26蛋白的缺失导致纤毛依赖性Hedgehog信号通路异常，阐明了其在胚胎发育中的关键作用。
4. **文献名称**: "Recombinant human TTC26 protein purification and its role in cell cycle regulation"
**作者**: Chen W, et al.
**摘要**: 描述了一种高效表达和纯化重组TTC26蛋白的方法，并发现该蛋白通过与细胞周期蛋白复合物相互作用调控G1/S期转换，影响细胞增殖。
*注：以上文献为模拟生成，实际研究需参考具体数据库(如PubMed)中的真实论文。*
TTC26. also known as Dyf13. is a conserved protein encoded by the TTC26 gene in humans. It belongs to the tetratricopeptide repeat (TPR) superfamily, characterized by structural motifs that mediate protein-protein interactions. Predominantly expressed in ciliated tissues, TTC26 localizes to the ciliary axoneme and basal bodies, playing a critical role in the assembly and function of motile cilia. Studies link TTC26 mutations to ciliopathies, including juvenile-onset hepatorenal fibrocystic diseases and respiratory defects due to impaired mucociliary clearance.
Biochemically, TTC26 interacts with components of the intraflagellar transport (IFT) machinery, particularly IFT-B complexes, facilitating cargo transport during ciliogenesis. Its dysfunction disrupts ciliary motility, leading to abnormal fluid movement in organs like lungs, liver, and kidneys. Recombinant human TTC26 protein, typically produced in bacterial or eukaryotic expression systems, serves as a tool for structural studies, antibody development, and functional assays to investigate cilia-related pathways. Research also explores its potential as a biomarker for ciliopathies or a therapeutic target. Ongoing studies aim to delineate its precise molecular interactions and regulatory mechanisms in ciliary disorders.
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