| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC25 |
| Uniprot No | Q96NG3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-672 aa |
| 活性数据 | MSDPEGETLR STFPSYMAEG ERLYLCGEFS KAAQSFSNAL YLQDGDKNCL VARSKCFLKM GDLERSLKDA EASLQSDPAF CKGILQKAET LYTMGDFEFA LVFYHRGYKL RPDREFRVGI QKAQEAINNS VGSPSSIKLE NKGDLSFLSK QAENIKAQQK PQPMKHLLHP TKGEPKWKAS LKSEKTVRQL LGELYVDKEY LEKLLLDEDL IKGTMKGGLT VEDLIMTGIN YLDTHSNFWR QQKPIYARER DRKLMQEKWL RDHKRRPSQT AHYILKSLED IDMLLTSGSA EGSLQKAEKV LKKVLEWNKE EVPNKDELVG NLYSCIGNAQ IELGQMEAAL QSHRKDLEIA KEYDLPDAKS RALDNIGRVF ARVGKFQQAI DTWEEKIPLA KTTLEKTWLF HEIGRCYLEL DQAWQAQNYG EKSQQCAEEE GDIEWQLNAS VLVAQAQVKL RDFESAVNNF EKALERAKLV HNNEAQQAII SALDDANKGI IRELRKTNYV ENLKEKSEGE ASLYEDRIIT REKDMRRVRD EPEKVVKQWD HSEDEKETDE DDEAFGEALQ SPASGKQSVE AGKARSDLGA VAKGLSGELG TRSGETGRKL LEAGRRESRE IYRRPSGELE QRLSGEFSRQ EPEELKKLSE VGRREPEELG KTQFGEIGET KKTGNEMEKE YE |
| 分子量 | 76.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC25蛋白的3篇代表性文献示例(注：以下内容为模拟文献，实际研究中可能需查阅具体数据库补充)：
1. **文献名称**: *"Structural and functional characterization of TTC25 in ciliary dynein assembly"*
**作者**: Smith A, et al.
**摘要**: 研究解析了人TTC25蛋白的晶体结构，揭示其作为微管动力蛋白复合体(dynein)的辅助因子，参与纤毛轴丝组装的功能机制，并通过体外重组实验验证其与动力蛋白轻链的相互作用。
2. **文献名称**: *"TTC25 mutations cause primary ciliary dyskinesia with disrupted ciliary motility"*
**作者**: Chen L, et al.
**摘要**: 该研究通过全外显子测序发现TTC25基因突变与原发性纤毛运动障碍(PCD)相关，重组人TTC25蛋白的功能缺失实验证实其在纤毛搏动调节中起关键作用，突变导致患者呼吸道纤毛运动异常。
3. **文献名称**: *"Recombinant human TTC25 expression in HEK293 cells: implications for ciliopathy screening"*
**作者**: Wang Y, et al.
**摘要**: 报道了利用HEK293细胞系统高效表达重组人TTC25蛋白的方法，并建立基于该蛋白的体外筛选平台，用于评估药物对纤毛相关疾病的潜在治疗作用。
实际文献建议通过PubMed或Google Scholar以“TTC25 protein”、“human TTC25 recombinant”等关键词检索获取。
TTC25 (Tetratricopeptide repeat domain 25) is a human protein characterized by its conserved tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions. It is primarily associated with ciliary function, particularly in the assembly and regulation of dynein motor complexes within motile cilia and flagella. These structures are critical for cellular motility, fluid movement, and sensory signaling. Studies link TTC25 to the cytoplasmic preassembly of dynein arms before their transport into cilia, a process essential for proper ciliary beating. Dysfunction of TTC25 has been implicated in primary ciliary dyskinesia (PCD), a genetic disorder marked by recurrent respiratory infections, infertility, and situs inversus due to impaired mucociliary clearance.
Recombinant human TTC25 protein is engineered using expression systems like *E. coli* or mammalian cell cultures, enabling controlled study of its biochemical properties and interactions. Its recombinant form facilitates research into ciliopathies, dynein complex dynamics, and intracellular trafficking mechanisms. Recent investigations also explore its potential role beyond cilia, including microtubule organization and cell cycle regulation. As a research tool, recombinant TTC25 aids in dissecting molecular pathways underlying PCD and developing targeted therapies. Standardized production of this protein supports antibody development, structural studies (e.g., X-ray crystallography), and functional assays to validate genetic mutations in clinical diagnostics.
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