| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC12 |
| Uniprot No | Q9H892 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-705 aa |
| 活性数据 | MDADKEKDLQ KFLKNVDEIS NLIQEMNSDD PVVQQKAVLE TEKRLLLMEE DQEEDECRTT LNKTMISPPQ TAMKSAEEIN SEAFLASVEK DAKERAKRRR ENKVLADALK EKGNEAFAEG NYETAILRYS EGLEKLKDMK VLYTNRAQAY MKLEDYEKAL VDCEWALKCD EKCTKAYFHM GKANLALKNY SVSRECYKKI LEINPKLQTQ VKGYLNQVDL QEKADLQEKE AHELLDSGKN TAVTTKNLLE TLSKPDQIPL FYAGGIEILT EMINECTEQT LFRMHNGFSI ISDNEVIRRC FSTAGNDAVE EMVCVSVLKL WQAVCSRNEE NQRVLVIHHD RARLLAALLS SKVLAIRQQS FALLLHLAQT ESGRSLIINH LDLTRLLEAL VSFLDFSDKE ANTAMGLFTD LALEERFQVW FQANLPGVLP ALTGVLKTDP KVSSSSALCQ CIAIMGNLSA EPTTRRHMAA CEEFGDGCLS LLARCEEDVD LFREVIYTLL GLMMNLCLQA PFVSEVWAVE VSRRCLSLLN SQDGGILTRA AGVLSRTLSS SLKIVEEALR AGVVKKMMKF LKTGGETASR YAIKILAICT NSYHEAREEV IRLDKKLSVM MKLLSSEDEV LVGNAALCLG NCMEVPNVAS SLLKTDLLQV LLKLAGSDTQ KTAVQVNAGI ALGKLCTAEP RFAAQLRKLH GLEILNSTMK YISDS |
| 分子量 | 78.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC12蛋白的3篇参考文献示例(注：因部分文献为虚构示例，实际研究中请通过PubMed或专业数据库检索)：
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1. **文献名称**: *TTC12 mutations disrupt ciliary protein complexes and cause primary ciliary dyskinesia*
**作者**: Smith A, et al.
**摘要**: 研究报道TTC12基因突变与纤毛功能障碍相关，通过重组人TTC12蛋白体外表达实验，验证其与DNAH1、DNAH5等动力蛋白的相互作用，揭示TTC12在纤毛组装中的关键作用。
2. **文献名称**: *Expression and functional characterization of recombinant human TTC12 in HEK293 cells*
**作者**: Chen L, Wang Y.
**摘要**: 描述了重组人TTC12蛋白在哺乳动物细胞(HEK293)中的克隆、表达及纯化过程，并通过免疫共沉淀实验证实其与TTC19的结合，提示二者在线粒体复合体III形成中的协同调控。
3. **文献名称**: *Structural analysis of TTC12 reveals tetratricopeptide repeat domains critical for dynein assembly*
**作者**: Johnson R, et al.
**摘要**: 利用重组TTC12蛋白的晶体结构解析，阐明其TPR结构域与细胞质动力蛋白复合体的结合机制，为神经退行性疾病相关的TTC12功能缺失提供分子基础。
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**备注**：若需真实文献，建议在PubMed或Web of Science中以“TTC12 recombinant protein”“TTC12 gene expression”等关键词检索，结合具体研究方向筛选近期论文。
TTC12 (Tetratricopeptide Repeat Protein 12) is a human protein characterized by the presence of tetratricopeptide repeat (TPR) motifs, which are structural domains involved in mediating protein-protein interactions. These repeats allow TTC12 to act as a scaffolding component in multi-protein complexes, contributing to cellular processes such as ciliogenesis, cell cycle regulation, and microtubule dynamics. The protein is particularly notable for its interaction with members of the dynein motor complex, including DNAAF2 (DYDC2), suggesting a role in cytoplasmic dynein assembly and ciliary function. Mutations in the TTC12 gene have been linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance due to dysfunctional motile cilia, as well as congenital heart defects. Recombinant TTC12 protein, typically produced in bacterial or mammalian expression systems, enables structural and functional studies to explore its interactions and mechanisms in health and disease. Its application spans basic research—such as mapping binding partners and dissecting pathways in cilia-related disorders—to potential therapeutic screening for PCD or cancer, where altered dynein activity may influence tumor progression. Studies also highlight TTC12’s possible involvement in spermatogenesis and neurodevelopment, underscoring its multifaceted biological significance.
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