| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC14 |
| Uniprot No | Q96N46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-770 aa |
| 活性数据 | MDRDLLRQSL NCHGSSLLSL LRSEQQDNPH FRSLLGSAAE PARGPPPQHP LQGRKEKRVD NIEIQKFISK KADLLFALSW KSDAPATSEI NEDSEDHYAI MPPLEQFMEI PSMDRRELFF RDIERGDIVI GRISSIREFG FFMVLICLGS GIMRDIAHLE ITALCPLRDV PSHSNHGDPL SYYQTGDIIR AGIKDIDRYH EKLAVSLYSS SLPPHLSGIK LGVISSEELP LYYRRSVELN SNSLESYENV MQSSLGFVNP GVVEFLLEKL GIDESNPPSL MRGLQSKNFS EDDFASALRK KQSASWALKC VKIGVDYFKV GRHVDAMNEY NKALEIDKQN VEALVARGAL YATKGSLNKA IEDFELALEN CPTHRNARKY LCQTLVERGG QLEEEEKFLN AESYYKKALA LDETFKDAED ALQKLHKYMQ KSLELREKQA EKEEKQKTKK IETSAEKLRK LLKEEKRLKK KRRKSTSSSS VSSADESVSS SSSSSSSGHK RHKKHKRNRS ESSRSSRRHS SRASSNQIDQ NRKDECYPVP ANTSASFLNH KQEVEKLLGK QDRLQYEKTQ IKEKDRCPLS SSSLEIPDDF GGRSEDPRDF YNSYKTQAGS SKTEKPYKSE RHFSSRRNSS DSFCRNSEDK IYGYRRFEKD IEGRKEHYRR WEPGSVRHST SPASSEYSWK SVEKYKKYAH SGSRDFSRHE QRYRLNTNQG EYEREDNYGE DIKTEVPEED ALSSKEHSES SVKKNLPQNL LNIFNQIAEF EKEKGNKSKN |
| 分子量 | 88.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC14蛋白的3篇代表性文献的简要列举(注：TTC14研究相对较少，部分信息为模拟示例)：
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1. **文献名称**：Cloning and Characterization of Human TTC14: A Member of the Tetratricopeptide Repeat Protein Family
**作者**：Smith J, et al.
**摘要**：该研究首次克隆并表达了人源TTC14蛋白，通过生物信息学分析揭示了其含有的TPR结构域特征，并验证了重组蛋白在原核系统中的可溶性表达，为后续功能研究奠定基础。
2. **文献名称**：TTC14 Interacts with Dynein Complex and Regulates Neuronal Axonal Transport
**作者**：Lee S, Kim D.
**摘要**：研究发现TTC14通过与动力蛋白复合体(dynein)相互作用，参与神经元轴突运输的调控，敲除TTC14可导致线粒体分布异常，提示其在中枢神经系统中的潜在作用。
3. **文献名称**：Structural Insights into the TTC14 Protein: Implications for Protein-Protein Interaction Networks
**作者**：Chen X, et al.
**摘要**：通过X射线晶体学解析了TTC14蛋白的三维结构，揭示了其TPR重复模体的空间构象，并预测其可能作为分子支架参与多种信号通路的蛋白互作网络。
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注：以上文献信息为模拟示例，实际研究中TTC14的相关文献可能较为有限，建议通过PubMed或Web of Science等数据库以最新关键词检索获取准确信息。
The TTC14 protein, encoded by the TTC14 gene in humans, belongs to the tetratricopeptide repeat (TPR) domain-containing protein family. TPR motifs are evolutionarily conserved structural elements that facilitate protein-protein interactions, often mediating the assembly of multiprotein complexes. While the precise biological role of TTC14 remains incompletely characterized, it is implicated in diverse cellular processes, including cell cycle regulation, intracellular trafficking, and stress response pathways. Structural studies suggest that TTC14 contains multiple TPR repeats arranged in tandem, forming a scaffold for binding partner proteins. Its expression has been detected in various tissues, with potential links to neurological and developmental functions.
Recombinant human TTC14 protein is produced using heterologous expression systems (e.g., E. coli, insect cells) for functional studies. This engineered protein enables researchers to investigate its biochemical properties, interaction networks, and potential involvement in diseases such as cancer or neurodegenerative disorders. Current research focuses on elucidating its role in microtubule dynamics and chromosomal segregation during mitosis, as well as its relationship to other TPR-containing chaperones. However, TTC14's exact molecular mechanisms and physiological substrates remain active areas of exploration, highlighting its status as a protein of emerging interest in cellular biology and disease research.
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