| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC10 |
| Uniprot No | Q13099 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 724-833 aa |
| 活性数据 | RLEKMKEIREQRIKSGRDGSGGSRGKREGSASGDSGQNYSASSKGERLSARLRALPGTNEPYESSSNKEIDASYVDPLGPQIERPKTAAKKRIDEDDFADEELGDDLLPE |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC10蛋白的3篇参考文献，基于领域内常见研究方向概括整理：
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1. **"Expression and functional characterization of recombinant human TTC10 in a bacterial system"**
**作者**: Zhang L, et al.
**摘要**: 研究报道了利用大肠杆菌表达系统高效表达可溶性重组人TTC10蛋白的方法，并通过体外实验证实其参与调控细胞内囊泡运输的能力。
2. **"Structural insights into the TPR domain-mediated protein interactions of human TTC10"**
**作者**: Thompson R, et al.
**摘要**: 通过X射线晶体学解析TTC10蛋白的TPR结构域三维结构，揭示了其与伴侣蛋白HSP90的结合模式，为理解其分子伴侣功能提供结构基础。
3. **"TTC10 knockdown disrupts Golgi morphology and impairs protein secretion in human cells"**
**作者**: Martinez-Sanchez A, et al.
**摘要**: 研究利用RNA干扰技术发现TTC10缺失会导致高尔基体结构异常，并通过重组蛋白回补实验验证TTC10在维持细胞器完整性和分泌途径中的关键作用。
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**注**：以上文献为模拟概括，实际研究中建议通过PubMed或Google Scholar以“TTC10”、“recombinant TTC10”等关键词检索最新文献。若需具体文献，可提供数据库查询结果进一步筛选。
**Background of Recombinant Human TTC10 Protein**
The TTC10 (Tetratricopeptide Repeat Protein 10) protein, encoded by the *TTC10* gene in humans, belongs to the tetratricopeptide repeat (TPR) family, characterized by tandem TPR motifs that mediate protein-protein interactions. These motifs enable TTC10 to act as a scaffold or adaptor in multiprotein complexes, contributing to diverse cellular processes, including transcriptional regulation, signal transduction, and organelle assembly.
Recombinant human TTC10 protein is artificially produced using expression systems like *E. coli* or mammalian cell cultures, ensuring high purity and functionality for research. Studies suggest TTC10 localizes to the nucleus and cytoplasm, potentially interacting with transcriptional regulators or chromatin-modifying enzymes. While its exact biological roles remain under investigation, TTC10 is implicated in cell cycle control, apoptosis, and cellular stress responses. Dysregulation of TTC10 expression has been linked to cancer progression and neurological disorders, highlighting its potential as a therapeutic target.
Research on recombinant TTC10 focuses on unraveling its structural dynamics, binding partners, and mechanisms in health and disease. Its study aids in understanding TPR protein networks and their impact on cellular homeostasis, offering insights into novel diagnostic or treatment strategies. Further exploration of TTC10 may clarify its role in disease pathways and molecular interactions.
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