| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSK1B |
| Uniprot No | Q9BXA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367 aa |
| 活性数据 | MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST METEEGPPQQ PPETRAQ |
| 分子量 | 41.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSSK1B蛋白的参考文献示例(虚构内容，仅作参考)：
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1. **文献名称**: *Expression and functional characterization of recombinant human TSSK1B in mammalian cells*
**作者**: Wang, H., et al.
**摘要**: 本研究成功在大肠杆菌和HEK293细胞中表达并纯化了重组人TSSK1B蛋白，通过体外激酶实验证实其磷酸化底物的活性，并揭示其在精子尾部发育中的潜在作用。
2. **文献名称**: *Structural insights into TSSK1B kinase domain via recombinant protein crystallography*
**作者**: Kumar, R., et al.
**摘要**: 利用重组表达技术获得高纯度TSSK1B激酶结构域，并解析其晶体结构，发现其ATP结合口袋的独特构象，为设计靶向抑制剂提供结构基础。
3. **文献名称**: *TSSK1B as a therapeutic target in male infertility: Screening with recombinant protein-based assays*
**作者**: Smith, J., et al.
**摘要**: 通过重组TSSK1B蛋白构建高通量药物筛选平台，鉴定出若干小分子抑制剂，验证其在改善精子活力及受精能力中的效果，支持其作为不育症治疗靶点的潜力。
4. **文献名称**: *Optimization of TSSK1B recombinant expression in E. coli and activity analysis*
**作者**: Chen, L., et al.
**摘要**: 系统优化TSSK1B在大肠杆菌中的可溶性表达条件，建立一步法镍柱亲和纯化流程，并通过质谱分析确认磷酸化修饰位点，为功能研究提供可靠工具蛋白。
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注：上述文献为模拟内容，实际研究中请通过PubMed、Web of Science等平台检索真实论文。
**Background of Recombinant Human TSSK1B Protein**
TSSK1B (Testis-Specific Serine Kinase 1B), also known as STK22B, is a member of the testis-specific serine/threonine kinase family, predominantly expressed in post-meiotic male germ cells. This protein plays a critical role in spermatogenesis, particularly during late-stage sperm development, including sperm tail formation, motility, and fertilization competence. Structurally, TSSK1B contains a conserved catalytic kinase domain and unique N-terminal extensions, distinguishing it from other kinases.
Recombinant human TSSK1B protein is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its recombinant form enables investigation into substrate interactions, enzymatic activity, and post-translational modifications (e.g., autophosphorylation). Research highlights its involvement in signaling pathways regulating chromatin condensation, acrosome formation, and sperm capacitation.
TSSK1B’s testis-specific expression makes it a potential target for male contraceptive development. Aberrant TSSK1B expression or mutations are also linked to male infertility, underscoring its clinical relevance. Recent studies explore its role beyond reproduction, including potential oncogenic roles in certain cancers. However, its full physiological scope and regulatory mechanisms remain under active investigation.
In summary, recombinant TSSK1B serves as a vital tool for deciphering molecular mechanisms in spermatogenesis and developing therapeutic strategies for reproductive disorders.
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