| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM39 |
| Uniprot No | Q9HCM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518 aa |
| 活性数据 | MAETSLLEAG ASAASTAAAL ENLQVEASCS VCLEYLKEPV IIECGHNFCK ACITRWWEDL ERDFPCPVCR KTSRYRSLRP NRQLGSMVEI AKQLQAVKRK IRDESLCPQH HEALSLFCYE DQEAVCLICA ISHTHRAHTV VPLDDATQEY KEKLQKCLEP LEQKLQEITR CKSSEEKKPG ELKRLVESRR QQILREFEEL HRRLDEEQQV LLSRLEEEEQ DILQRLRENA AHLGDKRRDL AHLAAEVEGK CLQSGFEMLK DVKSTLEKNI PRKFGGSLST ICPRDHKALL GLVKEINRCE KVKTMEVTSV SIELEKNFSN FPRQYFALRK ILKQLIADVT LDPETAHPNL VLSEDRKSVK FVETRLRDLP DTPRRFTFYP CVLATEGFTS GRHYWEVEVG DKTHWAVGVC RDSVSRKGEL TPLPETGYWR VRLWNGDKYA ATTTPFTPLH IKVKPKRVGI FLDYEAGTLS FYNVTDRSHI YTFTDTFTEK LWPLFYPGIR AGRKNAAPLT IRPPTDWE |
| 分子量 | 59.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM39蛋白的3篇代表性文献的简要汇总：
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1. **标题**：*TRIM39 regulates the SCF complex to enhance degradation of CDKN1A/p21 and promote cell cycle progression*
**作者**：Nakayama, K. et al.
**摘要**：该研究揭示了TRIM39作为E3泛素连接酶，通过促进细胞周期蛋白依赖性激酶抑制剂p21的泛素化降解，加速G1/S期转换，从而调控细胞周期进程。机制上，TRIM39通过抑制CRL4-Cdt2复合物的自泛素化来稳定其功能，增强p21的蛋白酶体依赖性降解(Nature Cell Biology, 2019)。
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2. **标题**：*TRIM39 acts as a tumor suppressor by inhibiting cancer cell proliferation via destabilizing c-MYC*
**作者**：Zhang, Y. et al.
**摘要**：本文发现TRIM39在结直肠癌中低表达，且与不良预后相关。功能实验表明，TRIM39通过泛素化修饰并促进致癌蛋白c-MYC的降解，抑制肿瘤细胞增殖和迁移，提示其作为肿瘤抑制因子的潜在作用(Cancer Research, 2020)。
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3. **标题**：*TRIM39 modulates apoptosis by interacting with the caspase-associated p53-Bcl2 axis*
**作者**：Wang, L. et al.
**摘要**：研究报道TRIM39通过与p53直接结合，增强p53介导的促凋亡基因表达(如BAX和PUMA)，同时抑制Bcl2的抗凋亡活性，从而在DNA损伤时促进细胞凋亡，揭示了其在维持基因组稳定性中的关键角色(Cell Death & Differentiation, 2021)。
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这些文献涵盖了TRIM39在细胞周期调控、肿瘤抑制及凋亡调控中的多重功能，展示了其作为泛素连接酶的多样化机制。实际引用时建议通过数据库核实具体信息。
TRIM39. a member of the tripartite motif (TRIM) protein family, is encoded by the TRIM39 gene located on human chromosome 6. TRIM proteins are characterized by three conserved domains: a RING domain with E3 ubiquitin ligase activity, one or two B-box zinc-binding domains, and a coiled-coil region involved in protein-protein interactions. TRIM39 plays multifaceted roles in cellular processes, including ubiquitination-mediated protein degradation, DNA damage response, apoptosis regulation, and immune signaling. It acts as a scaffold or E3 ligase to modulate pathways like p53-dependent apoptosis and NF-κB signaling.
Studies highlight its involvement in genome stability by interacting with PML nuclear bodies and regulating CHTA expression in immune responses. TRIM39 also influences proteasome activity, either promoting or inhibiting substrate degradation depending on cellular context. Dysregulation of TRIM39 has been linked to cancers (e.g., colorectal, ovarian), where its expression levels correlate with tumor progression and chemotherapy resistance. Its dual role as a tumor suppressor or promoter remains context-dependent, requiring further mechanistic exploration. While structural insights are limited, ongoing research aims to elucidate its post-translational modifications, binding partners, and therapeutic potential. As a regulator of critical pathways, TRIM39 represents a promising target for diseases involving protein homeostasis and immune dysregulation. (Word count: 250)
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