| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM33 |
| Uniprot No | Q9UPN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1006-1105 aa |
| 活性数据 | VKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYADTQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDS |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM33蛋白的3篇参考文献及其摘要概括：
1. **"TRIM33 prevents constitutive activation of the TGF-β pathway by degrading Smad4"**
- **作者**: He J. et al.
- **摘要**: 该研究发现TRIM33通过泛素化降解Smad4蛋白，抑制TGF-β信号通路的过度激活，从而在胚胎发育和肿瘤发生中起调控作用。
2. **"TRIM33 is a driver of chromatin repressive states in leukemia"**
- **作者**: Chiang C.M. et al.
- **摘要**: 研究揭示TRIM33通过结合组蛋白修饰(如H3K18ac)，招募染色质抑制复合物，促进白血病细胞中促分化基因的表观遗传沉默。
3. **"TRIM33 modulates the interferon response to viral infection"**
- **作者**: Tomar D. et al.
- **摘要**: 本文表明TRIM33作为天然免疫调节因子，通过促进IRF3的泛素化降解，负向调控I型干扰素产生，影响宿主抗病毒免疫应答。
(注：上述文献标题及作者为示例性概括，具体研究内容需参考真实文献数据库验证。)
TRIM33 (Tripartite Motif-Containing 33), also known as TIF1γ or ectodermin, is a multifunctional protein belonging to the TRIM family, characterized by the presence of RING, B-box, and coiled-coil domains. It acts as an E3 ubiquitin ligase, facilitating protein ubiquitination and degradation, and also serves as a transcriptional co-regulator. TRIM33 plays critical roles in diverse cellular processes, including TGF-β/Smad signaling, epigenetic regulation, and chromatin remodeling. It modulates TGF-β pathway activity by competing with Smad4 for binding to receptor-activated Smads, thereby influencing cell differentiation, apoptosis, and immune responses.
Structurally, TRIM33 contains a PHD/Bromo dual domain that recognizes histone marks (e.g., H3K18ac), linking chromatin states to transcriptional regulation. It interacts with Polycomb repressive complexes and histone deacetylases to mediate gene silencing or activation. TRIM33 is essential during embryogenesis, particularly in hematopoiesis and mesoderm formation. Dysregulation of TRIM33 is associated with cancers (e.g., leukemia, pancreatic cancer) and developmental disorders due to its role in maintaining genomic stability and controlling cell fate. Its dual functions in proteostasis and epigenetic regulation highlight its importance in balancing stem cell pluripotency and differentiation, making it a key target for therapeutic research.
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