| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM35 |
| Uniprot No | Q9UPQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493 aa |
| 活性数据 | MERSPDVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRGCVSRCWE VQVSPTCPVC KDRASPADLR TNHTLNNLVE KLLREEAEGA RWTSYRFSRV CRLHRGQLSL FCLEDKELLC CSCQADPRHQ GHRVQPVKDT AHDFRAKCRN MEHALREKAK AFWAMRRSYE AIAKHNQVEA AWLEGRIRQE FDKLREFLRV EEQAILDAMA EETRQKQLLA DEKMKQLTEE TEVLAHEIER LQMEMKEDDV SFLMKHKSRK RRLFCTMEPE PVQPGMLIDV CKYLGSLQYR VWKKMLASVE SVPFSFDPNT AAGWLSVSDD LTSVTNHGYR VQVENPERFS SAPCLLGSRV FSQGSHAWEV ALGGLQSWRV GVVRVRQDSG AEGHSHSCYH DTRSGFWYVC RTQGVEGDHC VTSDPATSPL VLAIPRRLRV ELECEEGELS FYDAERHCHL YTFHARFGEV RPYFYLGGAR GAGPPEPLRI CPLHISVKEE LDG |
| 分子量 | 56.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRIM35蛋白的假设参考文献示例，涵盖其功能、结构及疾病关联：
1. **Zhang, Y. et al. "TRIM35 mediates protection against viral infection by promoting ubiquitination of MAVS"**
该研究发现TRIM35作为E3泛素连接酶，通过催化MAVS蛋白的K63连接泛素化，增强I型干扰素信号通路活性，从而有效抑制RNA病毒的复制。
2. **Li, H. et al. "TRIM35 suppresses hepatocellular carcinoma progression by modulating AKT/mTOR signaling"**
研究显示TRIM35在肝癌组织中表达显著降低，其过表达可抑制AKT/mTOR通路，诱导癌细胞凋亡并抑制增殖与转移，提示其作为抑癌基因的潜在作用。
3. **Wang, L. et al. "Structural insights into the E3 ligase activity of TRIM35 through RING domain dimerization"**
通过X射线晶体学分析，该研究揭示了TRIM35的RING结构域二聚化对其E3泛素连接酶活性至关重要，并提出结构域突变会削弱其对底物的泛素化能力。
4. **Chen, X. et al. "TRIM35 interacts with caspase-8 to promote TNFα-induced apoptosis"**
TRIM35被发现直接结合并激活caspase-8.增强TNFα触发的细胞凋亡，此机制可能被某些病毒蛋白靶向抑制，为病毒感染与宿主免疫互作提供新见解。
**注意**：上述文献为假设示例，实际研究中可能存在更多特异性细节或不同结论。建议通过PubMed或Google Scholar查询真实发表的文献。
TRIM35. also known as tripartite motif-containing protein 35. is a member of the TRIM family characterized by its conserved N-terminal tripartite motif (RING, B-box, and coiled-coil domains). This ubiquitously expressed protein plays diverse roles in cellular processes, including innate immunity, cell cycle regulation, and apoptosis. Unlike many TRIM proteins, TRIM35 lacks a canonical C-terminal substrate-binding domain, suggesting unique functional mechanisms.
A key feature of TRIM35 is its E3 ubiquitin ligase activity, mediated by the RING domain, which enables post-translational modification of target proteins through ubiquitination. It regulates signaling pathways such as NF-κB and MAPK, impacting inflammatory responses and stress adaptation. Studies highlight its involvement in antiviral immunity, where it interacts with RIG-I/MDA5 to modulate type I interferon production.
In cancer, TRIM35 exhibits dual roles: it acts as a tumor suppressor by promoting degradation of oncoproteins like PKM2 (pyruvate kinase M2) in hepatocellular carcinoma, yet it may facilitate tumor progression in certain contexts by stabilizing HIF-1α in hypoxia. Dysregulation of TRIM35 has been linked to metabolic reprogramming, metastasis, and chemoresistance. Its expression levels vary across cancer types, making it a potential prognostic marker or therapeutic target.
Despite growing interest, the full scope of TRIM35's interactome, tissue-specific functions, and regulatory mechanisms remain under investigation, necessitating further research to clarify its pathophysiological significance.
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