关键信息
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基因名
CHML
- 应用
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别名
CHM like; Chml; Choroideraemia like Protein; Choroideraemia-like Protein; Choroideremia like (Rab escort Protein 2); Choroideremia like; Choroideremia like Protein; Rab escort Protein 2; Rab Proteins geranylgeranyltransferase component A 2; RAE2_HUMAN; REP 2; REP-2; REP2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P26374
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表达区间
1-656aa
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氨基酸序列
MADNLPTEFDVVIIGTGLPESILAAACSRSGQRVLHIDSRSYYGGNWASFSFSGLLSWLKEYQQNNDIGEESTVVWQDLIHETEEAITLRKKDETIQHTEAFCYASQDMEDNVEEIGALQKNPSLGVSNTFTEVLDSALPEESQLSYFNSDEMPAKHTQKSDTEISLEVTDVEESVEKEKYCGDKTCMHTVSDKDGDKDESKSTVEDKADEPIRNRITYSQIVKEGRRFNIDLVSKLLYSQGLLIDLLIKSDVSRYVEFKNVTRILAFREGKVEQVPCSRADVFNSKELTMVEKRMLMKFLTFCLEYEQHPDEYQAFRQCSFSEYLKTKKLTPNLQHFVLHSIAMTSESSCTTIDGLNATKNFLQCLGRFGNTPFLFPLYGQGEIPQGFCRMCAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQRINAKYFIVEDSYLSEETCSNVQYKQISRAVLITDQSILKTDLDQQTSILIVPPAEPGACAVRVTELCSSTMTCMKDTYLVHLTCSSSKTAREDLESVVKKLFTPYTETEINEEELTKPRLLWALYFNMRDSSGISRSSYNGLPSNVYVCSGPDCGLGNEHAVKQAETLFQEIFPTEEFCPPPPNPEDIIFDGDDKQPEAPGTNNVVMAKLESSEESKNLESPEKHLQN
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分子量
100.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of CHML (Chaperonin-Containing Tetradecamer) recombinant protein has gained significant attention in the fields of molecular biology and biochemistry due to its crucial role in protein folding and cellular stress responses. CHML is a member of the chaperonin family, which assists in the proper folding of nascent polypeptides, thereby ensuring the correct functioning of proteins essential for various cellular processes. Abnormalities in protein folding can lead to diseases, including neurodegenerative disorders and certain cancers, making CHML a potential target for therapeutic interventions. Recent advancements in recombinant DNA technology have enabled researchers to produce large quantities of CHML, facilitating detailed structural and functional studies. Investigating the properties of CHML recombinant protein enhances our understanding of its mechanism of action and its interactions with other cellular components. Furthermore, these studies provide insights into the evolution of chaperonin systems across different organisms, highlighting their conserved roles in maintaining proteostasis. Overall, the exploration of CHML recombinant protein not only fills gaps in our knowledge regarding cellular physiology but also paves the way for innovative approaches in drug development and therapeutic strategies for mitigating diseases associated with protein misfolding.
