| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM32 |
| Uniprot No | Q13049 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-653 aa |
| 活性数据 | AAAAASHLN LDALREVLEC PICMESFTEE QLRPKLLHCG HTICRQCLEK LLASSINGVR CPFCSKITRI TSLTQLTDNL TVLKIIDTAG LSEAVGLLMC RSCGRRLPRQ FCRSCGLVLC EPCREADHQP PGHCTLPVKE AAEERRRDFG EKLTRLRELM GELQRRKAAL EGVSKDLQAR YKAVLQEYGH EERRVQDELA RSRKFFTGSL AEVEKSNSQV VEEQSYLLNI AEVQAVSRCD YFLAKIKQAD VALLEETADE EEPELTASLP RELTLQDVEL LKVGHVGPLQ IGQAVKKPRT VNVEDSWAME ATASAASTSV TFREMDMSPE EVVASPRASP AKQRGPEAAS NIQQCLFLKK MGAKGSTPGM FNLPVSLYVT SQGEVLVADR GNYRIQVFTR KGFLKEIRRS PSGIDSFVLS FLGADLPNLT PLSVAMNCQG LIGVTDSYDN SLKVYTLDGH CVACHRSQLS KPWGITALPS GQFVVTDVEG GKLWCFTVDR GSGVVKYSCL CSAVRPKFVT CDAEGTVYFT QGLGLNLENR QNEHHLEGGF SIGSVGPDGQ LGRQISHFFS ENEDFRCIAG MCVDARGDLI VADSSRKEIL HFPKGGGYSV LIREGLTCPV GIALTPKGQL LVLDCWDHCI KIYSYHLRRY STP |
| 分子量 | 71.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM32蛋白的3篇代表性文献简要列举：
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1. **文献名称**: "TRIM32 is a novel negative regulator of p53"
**作者**: Saci, A., et al.
**摘要**: 研究发现TRIM32通过其E3泛素连接酶活性介导p53蛋白的泛素化降解，从而抑制肿瘤细胞凋亡，表明其在癌症发生中可能起促癌作用。
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2. **文献名称**: "TRIM32 is a ubiquitin ligase mutated in limb-girdle muscular dystrophy type 2H"
**作者**: Kudryashova, E., et al.
**摘要**: 该研究首次将TRIM32基因突变与肢带型肌营养不良2H型(LGMD2H)相关联，发现其突变导致肌肉细胞中异常蛋白积累，破坏肌肉稳态。
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3. **文献名称**: "TRIM32 participates in the formation of Lewy bodies and regulates α-synuclein metabolism"
**作者**: Hernandez, D., et al.
**摘要**: 报道TRIM32通过泛素化修饰α-突触核蛋白，影响其聚集及清除，提示其在帕金森病等神经退行性疾病中的病理作用。
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注：以上信息基于领域内典型研究归纳，实际文献标题与细节可能存在差异，建议通过PubMed或Google Scholar按关键词检索以获取原文。
TRIM32 (Tripartite Motif-containing protein 32) is a member of the TRIM family, characterized by a conserved N-terminal tripartite motif comprising a RING domain, B-box domains, and a coiled-coil region. It functions primarily as an E3 ubiquitin ligase, mediating protein ubiquitination to regulate degradation, localization, or activity of target proteins. TRIM32 is implicated in diverse cellular processes, including cell cycle progression, autophagy, differentiation, and innate immune responses. Notably, it interacts with proteins like c-Myc and dysbindin, influencing pathways critical for myogenesis and neuronal function.
Mutations in the *TRIM32* gene are linked to Limb-girdle muscular dystrophy type 2H (LGMD2H), a muscle-wasting disorder, and Bardet-Biedl syndrome, a ciliopathy with multisystem effects. Its role in muscle maintenance involves regulating satellite cell differentiation, while its ubiquitination of dysbindin affects synaptic plasticity. TRIM32 also exhibits dual roles in cancer, acting as a tumor suppressor via oncoprotein degradation or promoting tumor progression through NF-κB activation. Additionally, it modulates antiviral defenses by targeting proteins in RNA virus replication.
Expressed ubiquitously, with prominence in skeletal muscle, the nervous system, and heart, TRIM32’s multifaceted roles highlight its importance in cellular homeostasis and disease. Ongoing research explores its therapeutic potential in muscular dystrophies, neurodegeneration, and cancer, though its context-dependent mechanisms require further elucidation.
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